Determination of norfloxacin, cipfloxacin, levofloxacin and moxifloxacin in urine by HPLC with a two-channel fluorescence detector

Journal of Science and Technology Pub Date : 2024-01-05 DOI:10.55401/jst.v6i4.2421

Nguyen Nguyen Truong, Nguyen Thi Minh Diep, Vu Anh Tuan

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Abstract

A sensitive HPLC-FLD method with two-channel fluorescence detection was established and validated for quantification of four fluoroquinolones (norfloxacin, ciprofloxacin, levofloxacin and moxifloxacin) in urine samples. The fluorescent detector was set at two-channel excitation and emission wavelengths: 280/445 nm (for NOR, CIP) and 290/500 nm (for LEVO, MOXI). The measurement protocol was in compatible with both of the sample treatment procedures including the liquid-liquid extraction (LLE) and solid-phase extraction (SPE). The method was well-validated with the limit of quantification: 0.027; 0.034; 0.024; 0.020 µg/mL (for LLE), and 0.028; 0.033; 0.024; 0.020 µg/mL (for SPE) for NOR, CIP, LEVO, MOXI, respectively. The method has been proved to be precise and accurate, with the relative standard deviation lower than 3% and the recovery ranging from 98.0%¸102.0%, for all fluoroquinolones. The proposed HPLC-FLD method provides an alternative approach for the simultaneous analysis of fluoroquinolones in urine. ® 2023 Journal of Science and Technology - NTTU

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利用双通道荧光检测器高效液相色谱法测定尿液中的诺氟沙星、环丙沙星、左氧氟沙星和莫西沙星

建立并验证了双通道荧光检测的高效液相色谱-荧光定量检测尿样中四种氟喹诺酮类药物（诺氟沙星、环丙沙星、左氧氟沙星和莫西沙星）的方法。荧光检测器的激发和发射波长分别为 280/445 nm（诺氟沙星、环丙沙星）和 290/500 nm（左氧氟沙星、莫西沙星）。测量方案与液-液萃取（LLE）和固相萃取（SPE）等两种样品处理程序兼容。该方法的定量限得到了很好的验证：NOR、CIP、LEVO、MOXI 的定量限分别为 0.027、0.034、0.024、0.020 µg/mL（LLE）和 0.028、0.033、0.024、0.020 µg/mL（SPE）。该方法对所有氟喹诺酮类药物的相对标准偏差均小于3%，回收率为98.0%¸102.0%，具有良好的精密度和准确度。该方法为尿液中氟喹诺酮类药物的同时分析提供了一种新的方法。® 2023 科技期刊 - NTTU

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Journal of Science and Technology

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