Histological findings for the absorption of small and large liposomes - the basis of future drug delivery and contrast media systems.

IF 1.2 4区 医学 Q4 DEVELOPMENTAL BIOLOGY
Roxana Florentina Şufaru, Cristinel Ionel Stan, Cătălina Anişoara Peptu, Liviu Ciprian Gavril, Dragoş Andrei Chiran, Dragoş Valentin Crauciuc, Eduard Gabriel Crauciuc, Mihaela Adela Iancu, Ruxandra Vatavu, Codrin Gabriel Lucasievici, Ana Maria Dumitrescu, Anca Sava
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Abstract

Background and objectives: The purpose of our study was to obtain and characterize carrier systems in different sizes that can affect oral absorption, since the mechanisms of liposome absorption are not yet fully understood. From stomach to the small intestine, liposomes can be gradually destroyed. Understanding the factors that affect oral absorption leads to developing safe and effective nanosystems to improve the oral delivery of therapeutics.

Materials and methods: We determined the efficiency of the absorption of small and large liposomes at the level of gingival mucosa, heart, liver, testicles, kidneys, and lungs, using frozen-section fluorescence microscopy, on rat tissues after liposomes administration. A number of 36 male rats were divided in four groups: control groups, A and C, consisted of six rats each and did not receive liposomes; two other groups, B and D, were the experimental ones, and consisted of 12 male rats each. The animals received small liposomes (75-76 nm) and large liposomes (80-87 nm), respectively, administered either by endogastric tube or intraperitoneal injection. After 24 hours, the animals were sacrificed, and we harvested the organs. We performed frozen sections and analyzed them with fluorescence microscopy.

Results: The frozen sections obtained from all organs revealed a higher absorption level of small liposomes in the testicles, liver, and gum, while the large liposomes had a greater affinity for the liver, with variations dependent on the route of administration.

Conclusions: Frozen-section fluorescence microscopy is a reliable technique for visualization of liposome absorption. Based on the size of these nanosystems, we revealed significant absorption for small liposomes in testicles, liver, heart, and gum, and for large liposomes mainly in the liver, compared with the control groups. The study advocates for the usage of liposomes for medical purposes, based on their absorption proprieties.

大小脂质体吸收的组织学研究结果--未来药物输送和造影剂系统的基础。
背景和目的:由于脂质体的吸收机制尚不完全清楚,我们研究的目的是获得不同大小的载体系统,并确定其特征,以影响口服吸收。从胃到小肠,脂质体会逐渐被破坏。了解影响口服吸收的因素有助于开发安全有效的纳米系统,改善治疗药物的口服给药:我们使用冷冻切片荧光显微镜测定了大鼠组织在服用脂质体后牙龈粘膜、心脏、肝脏、睾丸、肾脏和肺部对小脂质体和大脂质体的吸收效率。36 只雄性大鼠被分为四组:对照组 A 和 C 每组 6 只,不接受脂质体;另外两组 B 和 D 为实验组,每组 12 只。动物分别接受小脂质体(75-76 纳米)和大脂质体(80-87 纳米),通过胃内管或腹腔注射给药。24 小时后,动物被处死,我们收获了动物的器官。我们进行了冷冻切片,并用荧光显微镜进行了分析:从所有器官获得的冷冻切片显示,小脂质体在睾丸、肝脏和牙龈中的吸收水平较高,而大脂质体在肝脏中的亲和力较高,其变化取决于给药途径:结论:冷冻切片荧光显微镜是观察脂质体吸收的可靠技术。根据这些纳米系统的大小,我们发现与对照组相比,小脂质体在睾丸、肝脏、心脏和牙龈中的吸收显著,而大脂质体主要在肝脏中吸收。根据脂质体的吸收特性,这项研究提倡将脂质体用于医疗目的。
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来源期刊
CiteScore
1.70
自引率
20.00%
发文量
221
审稿时长
3-8 weeks
期刊介绍: Romanian Journal of Morphology and Embryology (Rom J Morphol Embryol) publishes studies on all aspects of normal morphology and human comparative and experimental pathology. The Journal accepts only researches that utilize modern investigation methods (studies of anatomy, pathology, cytopathology, immunohistochemistry, histochemistry, immunology, morphometry, molecular and cellular biology, electronic microscopy, etc.).
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