Resveratrol modulates the Nrf2/NF-κB pathway and inhibits TSLP-mediated atopic march.

IF 2.5 4区医学 Q3 ALLERGY

Allergologia et immunopathologia Pub Date : 2024-01-01 DOI:10.15586/aei.v52i1.963

Quan He, Weihua Liu, Zi Chen, Guangfei Wei, Jingxian Jiang, Liuchao Zhang, Linfu Zhou

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Abstract

Background: Resveratrol has been found to have anti-inflammatory and anti-allergic properties. The effects of resveratrol on thymic stromal lymphopoietin (TSLP)-mediated atopic march remain unclear.

Purpose: To explore the potential role of resveratrol in TSLP-mediated atopic march.

Methods: The atopic march mouse model was established by topical application of MC903 (a vitamin D3 analog). Following the treatment with resveratrol, airway resistance in mice was discovered by pulmonary function apparatus, and the number of total cells, neutrophils, and eosinophils in bronchoalveolar lavage fluid was counted. The histopathological features of pulmonary and ear skin tissues, inflammation, and cell infiltration were determined by hematoxylin and eosin staining. The messenger RNA (mRNA) levels of TSLP, immunoglobulin E, interleukin (IL)-4, IL-5, and IL-13 were measured by real-time quantitative polymerase chain reaction. The protein expression of nuclear factor kappa B (NF-κB)/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling-associated molecules (p-p65, p65, p-I kappa B kinase alpha (IκBα), IκBα, Nrf2, and TSLP) in lung and ear skin tissues were assessed by Western blot analysis.

Results: Resveratrol attenuated airway resistance and infiltration of total cells, eosinophils, and neutrophils in both lung and ear skin tissues. Resveratrol ameliorates serum inflammatory markers in allergic mice. Moreover, the phosphorylation levels of NF-κB pathway-related proteins were significantly reduced by administration of resveratrol in allergic lung and ear skin tissues. Similarly, the protein expression of TSLP in both lung and ear skin tissues was reduced by resveratrol, and Nrf2, a protector molecule, was increased with resveratrol treatment.

Conclusion: Resveratrol attenuates TSLP-reduced atopic march through ameliorating inflammation and cell infiltration in pulmonary and ear skin tissues by inhibiting the abnormal activation of NF-κB signaling pathway.

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白藜芦醇能调节 Nrf2/NF-κB 通路并抑制 TSLP 介导的特应性进展。

背景：白藜芦醇具有抗炎和抗过敏特性。目的：探讨白藜芦醇在胸腺基质淋巴细胞生成素（TSLP）介导的特应性进展中的潜在作用：方法：通过局部应用 MC903（维生素 D3 类似物）建立特应性进展小鼠模型。白藜芦醇治疗后，用肺功能仪检测小鼠气道阻力，并计算支气管肺泡灌洗液中的总细胞数、中性粒细胞数和嗜酸性粒细胞数。用苏木精和伊红染色法测定肺部和耳部皮肤组织的组织病理学特征、炎症和细胞浸润。实时定量聚合酶链反应测定了 TSLP、免疫球蛋白 E、白细胞介素（IL）-4、IL-5 和 IL-13 的信使 RNA（mRNA）水平。通过Western印迹分析评估了肺部和耳部皮肤组织中核因子卡巴B（NF-κB）/核因子红细胞2相关因子2（Nrf2）信号相关分子（p-p65、p65、p-I卡巴B激酶α（IκBα）、IκBα、Nrf2和TSLP）的蛋白表达：结果：白藜芦醇减轻了气道阻力以及肺和耳部皮肤组织中总细胞、嗜酸性粒细胞和中性粒细胞的浸润。白藜芦醇能改善过敏性小鼠血清中的炎症指标。此外，服用白藜芦醇后，过敏性肺部和耳部皮肤组织中 NF-κB 通路相关蛋白的磷酸化水平显著降低。同样，白藜芦醇也降低了TSLP在肺部和耳部皮肤组织中的蛋白表达，而保护分子Nrf2则在白藜芦醇治疗后有所增加：结论：白藜芦醇通过抑制NF-κB信号通路的异常激活，改善肺部和耳部皮肤组织的炎症和细胞浸润，从而减轻TSLP引起的特应性进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.