Current status and distribution of major RNA viruses infecting onion and garlic crops in Punjab, Pakistan

IF 0.7 4区农林科学 Q3 AGRONOMY

Emirates Journal of Food and Agriculture Pub Date : 2023-05-31 DOI:10.9755/ejfa.2023.3123

Muhammad Zubair Hamza, Muhammad Ashfaq, Hasan Riaz, Shafqat Saeed

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引用次数: 0

Abstract

Onion (Allium cepa L.) and garlic (Allium sativum L.) are significant bulbous vegetables well known for their kitchen and medicinal values across the globe including Pakistan. Allium crops are highly vulnerable to many notorious RNA viruses fit in different genera viz., Allexivirus, Potyvirus, Tospovirus and Carlavirus. These viruses are known to inflict colossal and havoc yield losses up to 50% in allium crops wherever they grown. The occurrence of allium viruses in Pakistan has already been reported, but in single crop and only a few studies describe their incidence and distribution. The study examined the current scenario and distribution of major RNA viruses infecting Allium crops in Punjab, Pakistan. Field surveys were carried out in 14 districts of Punjab province as well as capital territory Islamabad of Pakistan, for two consecutive growing seasons (2019 and 2020). The 3000 leaf samples collected, 1675 samples (Onion 791, Garlic 884) were found positive for RNA viruses by using specie specific DAS-ELISA and further confirmed through reverse transcriptase polymerase chain reaction (RT-PCR). The results revealed the presence of RNA viruses with overall disease incidence of 52.7% and the highest disease incidence was observed as Onion yellow dwarf virus (OYDV) 58% following Irish yellow spot virus (IYSV) 41.8% respectively in onion crop during 2019-2020. Similarly, in garlic crop overall disease incidence of RNA viruses was noticed as 58.9% and the highest disease incidence of OYDV was observed as 45%, following IYSV 32.4% and GarV-C 22.5% respectively during 2019-2020. Comparatively, disease incidence and geographical distribution of viruses was variable in surveyed regions of both crops during surveyed period, whereas OYDV and IYSV were most prevalent viruses infecting onion and garlic crops and GarV-C found only in garlic. The current study also concluded the importance to disseminate the information/awareness among the farmers regarding viral diseases and their management by using different strategies including use of resistant genotypes. Keywords: Allium crops; RNA viruses; ELISA; RT-PCR; Disease incidence; Virus distribution

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巴基斯坦旁遮普省感染洋葱和大蒜作物的主要 RNA 病毒的现状和分布情况

洋葱（Allium cepa L.）和大蒜（Allium sativum L.）是重要的球茎蔬菜，因其厨房和药用价值而闻名全球，巴基斯坦也不例外。葱类作物极易受到不同属的许多臭名昭著的 RNA 病毒的侵害，如 Allexivirus、Potyvirus、Tospovirus 和 Carlavirus。众所周知，无论在哪里种植，这些病毒都会给薤白作物造成高达 50%的巨大产量损失。巴基斯坦已经有关于薤白病毒发生的报道，但仅限于单一作物，只有少数研究描述了其发生率和分布情况。本研究调查了巴基斯坦旁遮普省感染薤白作物的主要 RNA 病毒的现状和分布情况。在巴基斯坦旁遮普省的 14 个县以及首都伊斯兰堡进行了连续两个生长季（2019 年和 2020 年）的实地调查。在收集到的 3000 份叶片样本中，有 1675 份样本（洋葱 791 份，大蒜 884 份）通过使用物种特异性 DAS-ELISA 发现 RNA 病毒呈阳性，并通过逆转录酶聚合酶链反应（RT-PCR）进一步确认。结果显示，2019-2020 年洋葱作物中存在 RNA 病毒，总发病率为 52.7%，最高发病率为洋葱黄矮病毒（OYDV）58%，爱尔兰黄斑病毒（IYSV）41.8%。同样，2019-2020 年期间，大蒜作物的 RNA 病毒总体发病率为 58.9%，OYDV 的发病率最高，为 45%，其次分别为 IYSV 32.4%和 GarV-C 22.5%。相比之下，在调查期间，两种作物的发病率和病毒地理分布在调查地区存在差异，而 OYDV 和 IYSV 是感染洋葱和大蒜作物最普遍的病毒，GarV-C 仅在大蒜中发现。本研究还得出结论，必须向农民传播有关病毒性疾病的信息/意识，并采用包括使用抗病基因型在内的不同策略来管理病毒性疾病。关键词葱属作物；RNA 病毒；ELISA；RT-PCR；病害发生率；病毒分布

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来源期刊

Emirates Journal of Food and Agriculture AGRONOMYFOOD SCIENCE & TECHNOLOGY&nb-FOOD SCIENCE & TECHNOLOGY

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.