Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells

IF 0.7 Q4 BIOLOGY

Nusantara Bioscience Pub Date : 2023-06-19 DOI:10.13057/nusbiosci/n150201

Mahendra Kumar Sahu, Ragini Yeeravalli, Amitava Das, Harit Jha

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Abstract

Abstract. Sahu MK, Yeeravalli R, Das A, Jha H. 2023. Secondary metabolites of rhizospheric fungal isolate Aspergillus carneus ABRF4 regulate the antibacterial and anti-proliferative activity against cancer cells. Nusantara Bioscience 15: 137-142. The medicinal capabilities of plants are influenced by soil chemistry, genotype, and climate. Many biotic and abiotic factors affect soil composition. Microorganisms constituting the soil microflora indicate a mutualistic relationship with plant rhizospheric region, and they play an important role in plant secondary metabolite production, yield, and efficacy. They are the major resources for structurally unique bioactive natural metabolites. The aim of the present study was to evaluate the bioactivity of the secondary metabolite extracted from the rhizospheric fungal isolate Aspergillus carneus ABRF4) isolated from the Achanakmar Biosphere Reserve, Chhattisgarh, India. The fungal secondary metabolites were extracted using several solvents by soxhlet extraction techniques. The crude and partially purified column fractions of A. carneus ABRF4 were characterized by Gas Chromatography-Mass Spectroscopy (GC-MS), Fourier Transform Infra-Red (FTIR) spectrum, and Thin Layer Chromatography (TLC). Results showed that the acetonitrile fraction had an antimicrobial activity with the variable zone of inhibition against human pathogens such as Bacillus circulans (MTCC-7906), Bacillus subtilis (MTCC 441), Staphylococcus aureus (MTCC-96), and Ralstonia eutropha (MTCC-2487). The crude extracts and the identified secondary metabolite, trans- 1,3-dimethyl-Cyclohexane, possess different anti-proliferative activity against human tissue-specific cancer cell lines, including breast cancer (MDA-MB-231, MDA-MB-468, and MCF-7), liver cancer (HepG2), lung cancer (A-549), and prostate cancer (DU-145) suggesting a potential therapeutic application of the isolated rhizospheric fungi.

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根瘤真菌分离物卡氏曲霉 ABRF4 的次级代谢产物调节对癌细胞的抗菌和抗增殖活性

摘要：Sahu MK, Yeeravalli R, Das A, Jha H. 2023.Sahu MK, Yeeravalli R, Das A, Jha H. 2023.根瘤真菌分离物Aspergillus carneus ABRF4的次级代谢产物调节对癌细胞的抗菌和抗增殖活性。Nusantara Bioscience 15: 137-142.植物的药用能力受土壤化学、基因型和气候的影响。许多生物和非生物因素都会影响土壤成分。构成土壤微生物区系的微生物与植物根瘤菌区系之间是一种互利关系，它们在植物次生代谢产物的生产、产量和药效方面发挥着重要作用。它们是结构独特的生物活性天然代谢物的主要资源。本研究旨在评估从印度恰蒂斯加尔邦 Achanakmar 生物圈保护区分离的根瘤真菌 ABRF4）中提取的次生代谢物的生物活性。真菌次生代谢物通过索氏提取技术使用多种溶剂进行提取。通过气相色谱-质谱（GC-MS）、傅立叶变换红外光谱（FTIR）和薄层色谱（TLC）对 A. carneus ABRF4 的粗提物和部分纯化柱馏分进行了表征。结果表明，乙腈馏分具有抗菌活性，对人类病原体（如环状芽孢杆菌（MTCC-7906）、枯草芽孢杆菌（MTCC 441）、金黄色葡萄球菌（MTCC-96）和赤霉菌（MTCC-2487））有不同的抑制区。粗提取物和鉴定出的次级代谢产物反式-1,3-二甲基环己烷对人体组织特异性癌细胞株具有不同的抗增殖活性，包括乳腺癌（MDA-MB-231、MDA-MB-468 和 MCF-7）、肝癌（HepG2）、肺癌（A-549）和前列腺癌（DU-145），这表明分离出的根瘤真菌具有潜在的治疗用途。

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来源期刊

Nusantara Bioscience BIOLOGY-

自引率

25.00%

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审稿时长

6 weeks