Evaluation of micronuclei in potentially malignant disorders and oral cancers: A cytological assay

IF 0.4 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Indian Academy of Oral Medicine and Radiology Pub Date : 2023-07-01 DOI:10.4103/jiaomr.jiaomr_40_23

Aishwarya Bhatnagar, Madhav Nagpal, Jyothi Kumar, Anamika Joshi, Sudhir Baroopal, Kailash Kewalia

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引用次数: 0

Abstract

Objective: The present study aims to evaluate the number of micronuclei (MN) in potentially malignant disorders (PMDs) and oral cancer and correlates them with the control group. Materials and Methods: Subjects included in the study were equally divided into four groups. Exfoliated cells were then obtained from the buccal mucosa using cytological brush. Smears were stained using rapid Pap stain. The smears were observed using a light microscope, and MN was counted out of 10 high-power fields and scored according to PE Tolbert (1992) criteria. Results: The mean MN count was found to be 0.23, 2.10, 3.03, and 3.57 in groups I, II, III, and IV, respectively. The overall intergroup comparison between the four groups was found to be statistically significant with a P value less than 0.05. Conclusion: This study gives a fair assessment that MN assay in exfoliated cells of oral epithelium can be a conducive biomarker while screening for PMDs of the oral cavity in doubtful lesions that are primarily misdiagnosed clinically.

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评估潜在恶性疾病和口腔癌中的微核：细胞学检测

研究目的本研究旨在评估潜在恶性疾病（PMD）和口腔癌中的微核（MN）数量，并将其与对照组进行比较。材料与方法：研究对象平均分为四组。然后用细胞学刷从颊粘膜获取脱落细胞。使用快速巴氏染色法对涂片进行染色。使用光学显微镜观察涂片，在 10 个高倍视野中对 MN 进行计数，并根据 PE Tolbert（1992 年）的标准进行评分。结果：I、II、III 和 IV 组的平均 MN 数分别为 0.23、2.10、3.03 和 3.57。四组之间的总体组间比较具有统计学意义，P 值小于 0.05。结论本研究对口腔上皮脱落细胞中的 MN 检测进行了中肯的评估，认为它可以作为一种生物标志物，用于筛查口腔 PMDs（主要是临床误诊的可疑病变）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Indian Academy of Oral Medicine and Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

32 weeks

期刊介绍： Journal of Indian Academy of Oral Medicine and Radiology (JIAOMR) (ISSN: Print - 0972-1363, Online - 0975-1572), an official publication of the Indian Academy of Oral Medicine and Radiology (IAOMR), is a peer-reviewed journal, published Quarterly , both in the form of hard copies (print version) as well as on the web (electronic version). The journal’s full text is available online at http://www.jiaomr.in. The journal allows free access (open access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository.