{"title":"Uji Daya Hambat Ekstrak Metanol Auricularia nigricans terhadap Candida spp.","authors":"Afina Indrawati, Amelya Dyah Pertiwi, Audry Ridho Ayuningtyias, Hillda Widianingtyas Subroto, Mufidhatul Nur Azizah, Tutik Handayani, Surahmaida Surahmaida, Kinanti Ayu Puji Lestari, Floreta Fiska Yuliarni","doi":"10.22437/biospecies.v16i2.27451","DOIUrl":null,"url":null,"abstract":"Candida was a fungus that can cause candidiasis infection. Candidiasis could occur of the skin, nails, mucous membranes and the gastrointestinal. The study aims to examine whether Auricularia nigricans methanol extract has the ability to inhibit the growth of Candida albicans, C. glabrata, and C. parapsilosis. Soxhletation was used as the extraction method, and the inhibitory test method involved diffusion paper disk and wells methods. The concentration variations used were 0.2 g/ml, 0.25 g/ml and 0.3 g/ml with 10% DMSO as a negative control. The research results obtained were that no inhibition zones were formed at concentrations of 0.2 g/ml, 0.25 g/ml and 0.3 g/ml so that they were included in the inactive category. Keywords: Auricularia nigricans, Candida, soxhlet method, methanol extract, paper disc and wells method. Abstrak Candida merupakan jamur golongan khamir yang dapat menyebabkan infeksi kandidiasis. Kandidiasis tersebut dapat terjadi pada kulit, kuku, membran mukosa dan saluran cerna. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metanol Auricularia nigricans dalam menghambat pertumbuhan C. albicans, C. glabrata dan C. parapsilosis. Metode ekstraksi yang digunakan adalah sokletasi selama 10 jam dan metode uji daya hambat antifunginya secara difusi cakram dan sumuran. Variasi konsentrasi yang digunakan adalah 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml dengan DMSO 10% sebagai kontrol negatif. Hasil penelitian yang diperoleh adalah tidak terbentuknya zona hambat pada konsentrasi 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml sehingga termasuk dalam kategori tidak aktif. Kata kunci: Auricularia nigricans, Candida, metode sokletasi, ekstrak metanol, metode difusi cakram dan sumuran.","PeriodicalId":31745,"journal":{"name":"Biospecies","volume":"9 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biospecies","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22437/biospecies.v16i2.27451","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Candida was a fungus that can cause candidiasis infection. Candidiasis could occur of the skin, nails, mucous membranes and the gastrointestinal. The study aims to examine whether Auricularia nigricans methanol extract has the ability to inhibit the growth of Candida albicans, C. glabrata, and C. parapsilosis. Soxhletation was used as the extraction method, and the inhibitory test method involved diffusion paper disk and wells methods. The concentration variations used were 0.2 g/ml, 0.25 g/ml and 0.3 g/ml with 10% DMSO as a negative control. The research results obtained were that no inhibition zones were formed at concentrations of 0.2 g/ml, 0.25 g/ml and 0.3 g/ml so that they were included in the inactive category. Keywords: Auricularia nigricans, Candida, soxhlet method, methanol extract, paper disc and wells method. Abstrak Candida merupakan jamur golongan khamir yang dapat menyebabkan infeksi kandidiasis. Kandidiasis tersebut dapat terjadi pada kulit, kuku, membran mukosa dan saluran cerna. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metanol Auricularia nigricans dalam menghambat pertumbuhan C. albicans, C. glabrata dan C. parapsilosis. Metode ekstraksi yang digunakan adalah sokletasi selama 10 jam dan metode uji daya hambat antifunginya secara difusi cakram dan sumuran. Variasi konsentrasi yang digunakan adalah 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml dengan DMSO 10% sebagai kontrol negatif. Hasil penelitian yang diperoleh adalah tidak terbentuknya zona hambat pada konsentrasi 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml sehingga termasuk dalam kategori tidak aktif. Kata kunci: Auricularia nigricans, Candida, metode sokletasi, ekstrak metanol, metode difusi cakram dan sumuran.