Genetic variability of populations of Pulsatilla species (Ranunculaceae) in the south of Yenisei Siberia

V. V. Zhivotov, K. K. Ryabova, I. E. Yamskikh, N. V. Stepanov, M. G. Kutsev
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Abstract

Pulsatilla is a genus of ephemeroid grasses characterized by high morphological diversity. The study assessed the genetic polymorphism of 9 populations represented by the species P orientali-sibirica Stepanov, P turczaninovii Krylov and their hybrid P. herba-somnii Stepanov, and P. multifida G. Prizel. As a result of amplification of genomic DNA with 4 ISSR primers, 61 DNA amplicons were obtained. The overall level of polymorphism is 98.36 %. The number of amplified DNA fragments, depending on the primer, varies from 11 (17899A) to 17 (17899 B, HB12). The maximum level of genetic variability was noted for hybrid populations. The coefficient of genetic subdivision (Gst) of the studied populations is 0.4718, which indicates a high level of their differentiation. The dendrogram divides the studied populations into 3 groups, each of which is represented by a separate species, while the hybrid population is combined with P. orientali-sibirica. Phylogenetic analysis of the studied populations combines P orientali-sibirica with P multifida and P turczaninovii with P. herba-somnii. These data demonstrate the intermediate position of the hybrid population of P. herba-somnii. In the study of hybrids, ISSRS are the most informative, since they are evenly distributed across the genome and can show similarities with two parent organisms, whereas chloroplasts are inherited, as a rule, through the maternal line.
叶尼塞西伯利亚南部白头翁属(Ranunculaceae)种群的遗传变异性
白头翁属(Pulsatilla)是一种昙花草属植物,具有形态多样性的特点。本研究评估了以 P orientali-sibirica Stepanov、P turczaninovii Krylov 及其杂交种 P. herba-somnii Stepanov 和 P. multifida G. Prizel 为代表的 9 个种群的遗传多态性。用 4 个 ISSR 引物扩增基因组 DNA 后,得到 61 个 DNA 扩增子。总体多态性水平为 98.36%。根据引物的不同,扩增 DNA 片段的数量从 11 个(17899A)到 17 个(17899B,HB12)不等。杂交种群的遗传变异水平最高。研究种群的遗传细分系数(Gst)为 0.4718,这表明它们的分化程度很高。树枝图将所研究的种群分为 3 组,每组代表一个单独的物种,而杂交种群则与 P. orientali-sibirica 结合在一起。所研究种群的系统发生分析将 P orientali-sibirica 与 P multifida 和 P turczaninovii 与 P. herba-somnii 结合在一起。这些数据证明了 P. herba-somnii 杂交种群的中间位置。在杂交种的研究中,ISSRS 的信息量最大,因为它们在基因组中分布均匀,能显示出与两个亲本生物的相似性,而叶绿体通常是通过母系遗传的。
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