Frequency of Immunohistochemical Marker GPC-3 Expression in Malignant Tumors

Pakistan Journal of Medical and Health Sciences Pub Date : 2023-07-06 DOI:10.53350/pjmhs2023176202

Noshaba Rahat, H. Shahzad

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Abstract

Objective: To determine the frequency of immunohistochemical marker GPC-3 expression in malignant tumours in a tertiary care hospital. Material & Methods: This cross sectional study was conducted at the department of Histopathology, Dr. Ziauddin Medical University and Hospital, north campus, Karachi, a total of 114 cases of malignant tumours were enrolled using consecutive sampling technique in a period of six months from 1st April, 2012 to 30th September, 2012. Patients of both genders and all age groups of histologically diagnosed case of hepatocellular carcinoma (HCC) metastatic tumours, germ cell tumours (GCT) and squamous Cell Carcinoma (SCC) were all included in the study. The patients with benign tumours, malignant lymphoma, brain tumours, breast tumours, colon carcinoma and malignant melanoma were excluded from the study. The study outcome was determined as frequency of positive GPC-3 expression. Results: A total of 114 cases were enrolled. Average age was 48.8 years with majority of cases (70.4%) were 40 years or above age. Male gender was slight dominance (53.5%). Squamous cell carcinoma was the most common (78.9%) followed by, germ cell tumours (10.5%) including (6.1%) yolk sac tumour and 4.3% Germinoma. Hepatocellular carcinoma was found in (6.1%) study cases. Most of the cases (45.6%) were moderately differentiated (SCC and HCC),followed by poorly (42.1%) and well differentiated (12.3%) grading. The frequency of positive GPC-3 expression was 25.4% in all malignant tumour in the current study. It is diagnostic and accuracy is more than other markers for definitive diagnosis and tp differenciate between primary and metastatic tumors. Conclusion: In conclusion out of 114 cases 25.4% of total cases show GCP-3 positively, among which GCT were 24.1% and HCC were 85.7% which is quite similar to other reported studies. Type of the tumour was found to be significantly associated with the age group above 40 years (P=0.000). There is significant association between type of tumour and GCP-3 expression (P=0.000). Keywords: Malignant Tumours, SCC, HCC GCT, GPC, GPC-3 expression

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恶性肿瘤中免疫组化标记物 GPC-3 的表达频率

目的确定一家三级医院恶性肿瘤中免疫组化标记物 GPC-3 的表达频率。材料与方法：这项横断面研究在卡拉奇北校区齐亚丁医科大学和医院组织病理学系进行，在 2012 年 4 月 1 日至 2012 年 9 月 30 日的 6 个月内，采用连续抽样技术共登记了 114 例恶性肿瘤病例。经组织学诊断为肝细胞癌（HCC）转移性肿瘤、生殖细胞瘤（GCT）和鳞状细胞癌（SCC）的男女老少患者均被纳入研究范围。良性肿瘤、恶性淋巴瘤、脑肿瘤、乳腺肿瘤、结肠癌和恶性黑色素瘤患者不在研究范围内。研究结果以 GPC-3 阳性表达的频率为准。研究结果共纳入 114 例病例。平均年龄为 48.8 岁，大多数病例（70.4%）年龄在 40 岁或以上。男性略占多数（53.5%）。最常见的是鳞状细胞癌（78.9%），其次是生殖细胞肿瘤（10.5%），包括卵黄囊肿瘤（6.1%）和生殖细胞瘤（4.3%）。肝细胞癌在研究病例中占 6.1%。大多数病例（45.6%）属于中度分化（SCC 和 HCC），其次是分化不良（42.1%）和分化良好（12.3%）。在本次研究中，所有恶性肿瘤中 GPC-3 阳性表达的比例为 25.4%。在明确诊断和区分原发性和转移性肿瘤方面，GPC-3 比其他标记物更具诊断性和准确性。结论总之，在 114 个病例中，25.4% 的病例显示 GCP-3 呈阳性，其中 GCT 占 24.1%，HCC 占 85.7%，这与其他报告的研究结果非常相似。研究发现，肿瘤类型与 40 岁以上年龄组有显著相关性（P=0.000）。肿瘤类型与GCP-3表达有明显相关性（P=0.000）。关键词：恶性肿瘤恶性肿瘤 SCC HCC GCT GPC GPC-3 表达

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Pakistan Journal of Medical and Health Sciences

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