TaqMan Genotyping Assay Method for Single Nucleotide Polymorphisms (SNPs) detection in promoter region of N-Acetyltransferase 2 (NAT2) gene

Abstract

The N-acetyltransferase 2 (NAT2) polymorphism in coding region has been studies intensively. However, there are limited studies for promoter region of NAT2 gene. Several reported study showed that the promoter region polymorphism of NAT2 gene is genotyped by PCR-sequencing approach. In this paper, we describe TaqMan based assays for the NAT2 polymorphism genotyping in promoter region with following SNP: rs4646243 [T>C], rs4646244 [T>A], rs4646267 [A>G], rs4345600 [A>G], and rs4646246 [A>G].  Our result showed a good separation cluster, trailing cluster and some mix cluster. TaqMan genotyping assay has shown a sensitivity and specificity to detect polymorphism in NAT2 promoter region.