Isolation and Molecular Confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen and is ubiquitous in nature and commonly found in soil, water, plants, and raw meat. It is major predominant spoilage bacteria in meat under refrigerated conditions. The study aimed to characterize the isolation, identification and molecular confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork. Study area, sampling, isolation, identification and molecular confirmation of Pseudomonas aeruginosa. A total 210 (n = 35 from each of the species) aerobically stored and frozen meat samples were collected from retail markets and slaughter houses in and around Chennai, India and analyzed. Among them, 22 isolates were confirmed as Pseudomonas aeruginosa by conventional, culture, biochemical and PCR assay targeting 16 SrRNA sequencing and amplification of rpoBgene. In this research, we successfully studied the occurrence, isolation, identification and molecular confirmation of Pseudomonas aeruginosa isolated from Chicken meat, Mutton and pork using conventional techniques.