An Ultra Structural Analysis of The Huh7 Hepatocellular Carcinoma Cell Line's Response to Conditioned and Co-Culture Media

Abstract

In this study, the gene expression of some cancer-related genes was measured to differentiate between two types of therapeutic media in which hepatocellular carcinoma (HuH7) were harvested. The first was a conditional medium in which umbilical cord mesenchymal stem cells were harvested then diluted by DMEM media (1: 1) and the second was Co-culture medium that mesenchymal stem cells and HuH7 cells (1:1) together were incubated for 3 days in a medium. The latter was called Co- culture medium. Comparison of the Results of gene expression of Surviving, PCNA, Beta-Catenin, Telomerase and VEGF by using real-time PCR in several four-four-time intervals (48-72-96 hrs.) showed that in conditioned media and co-culture media, hepatoma cell line (HuH7) expressed a significant down-regulation in Surviving (p< 0.001), PCNA (p<0.001), VEGF(p<0.001) and Beta- Catenin(p=0.022) but not significant in Telomerase (p= 0.617). In our study, the measure of gene expression at different time (ALT), Aspartate aminotransferase (AST), Albumin, Alkaline phosphate, gamma GT, alpha- fetoprotein when compared regarding time Results in a significant in Alpha-fetoprotein (p < 0.001), ALT (p < 0.001) and AST (p < 0.001) while the results of Albumin (p = 0.650), Alkaline phosphatase (p = 0.329) and Gamma GT (p = 0.844) were non-significant.