Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits

V. Evstifeev, F. М. Khusainov, S. I. Yakovlev, R. I. Shangaraev, V. I. Eremets
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Abstract

Specific antibodies against сhlamydia were detected using complement fixation test and сhlamydia genome was detected using polymerase chain reaction in pregnant rabbits experimentally infected with Chlamydia psittaci. The infected rabbits developed a fever and respiratory signs and the infection was confirmed by specific antibodies against сhlamydia detected in their blood and by abnormalities in rabbit kindling. Complement fixation test of paired rabbit sera revealed an increase in the titers of specific antibodies against сhlamydia, which on Day 7 post infection varied within 1:7.5; on Day 14, mean concentration was 1:40 and by Day 30 mean titer increased to 1:60. However, when pathological materials from the urogenital tract of the experimental animals were tested in polymerase chain reaction and in smear microscopy, it was impossible to confirm that there is an etiological link between сhlamydia and kindling problems in experimental animals. At the same time, molecular and genetic tests of internal organs (liver) sampled from stillborn baby rabbits revealed the сhlamydia genome, thus, proving сhlamydia involvement into the pathological kindling. Therefore, such a retrospective method as complement fixation test with a сhlamydia antigen is of high diagnostic value for lifetime сhlamydia diagnosis.
CFT 和 PCR 在诊断实验感染兔的鹦鹉热衣原体中的作用
利用补体固定试验检测了сhlamydia的特异性抗体,并利用聚合酶链反应检测了实验性感染鹦鹉热衣原体的怀孕兔子的сhlamydia基因组。受感染的兔子出现了发烧和呼吸道症状,通过在其血液中检测到针对сhlamydia的特异性抗体和兔子火鸡的异常现象确认了感染。对配对兔血清进行补体固定试验发现,针对сhlamydia的特异性抗体滴度有所上升,感染后第7天,滴度在1:7.5之间;第14天,平均浓度为1:40,到第30天,平均滴度上升到1:60。然而,在对实验动物泌尿生殖道的病理材料进行聚合酶链反应和涂片显微镜检测时,无法证实 сhlamydia 与实验动物的点燃问题之间存在病因学联系。与此同时,从死胎幼兔内脏(肝脏)取样进行的分子和基因检测发现了сhlamydia 基因组,从而证明сhlamydia 与病理雏鸡有关。因此,сhlamydia抗原补体固定试验这种回顾性方法对终生сhlamydia诊断具有很高的诊断价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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