ANTIHYPERURESEMIA ACTIVITY OF ETHANOL EXTRACTS AND FRACTIONS OF NIPAH LEAF FRONDS (NYPA FRUTICANS. WURMB)

Proceeding of International Conference on Science, Health, And Technology Pub Date : 2023-09-23 DOI:10.47701/icohetech.v4i1.3397

Danang Raharjo, Anita Dwi S, Muhammad Aminnulloh

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Abstract

Hyperuresemia is a metabolite syndrome characterised by high levels of uric acid in the blood. Hyperuresmia in advanced stages can cause gout or pyrexia (a disease that affects the joints and causes joint damage), kidney stones, kidney damage, and hypertension. The nipah plant (Nypa fruticans. Wurmb) is traditionally used in the treatment of stomach pain, diabetes, fever, toothache, uric acid reduction and headache medicine. In general, the content of secondary metabolite compounds from nipah plants includes flavonoids, phenols, tannins, terpenois, steroids, alkaloids and saponins. The purpose of this study was to determine the xanthine oxidase inhibitory activity of ethanol extracts and fractions of nipah leaves (Nypa fruticans. Wurmb). Extraction process using maceration method with 96% ethanol solvent, fractionation using liquid-liquid partition method with separating funnel. . Testing the inhibition of xanthine oxidase enzyme activity in vitro using UV/VIS spectrophotometer. The results showed that ethanol extract, water fraction, ethyl acetate fraction and n-hexane fraction showed inhibitory activity of xanthine oxidase enzyme with IC50 values of 47.558 ± 2.862; 18.755 ± 3.821; 3.543 ± 1.440 and 110.213 ± 1.577 ppm, respectively. From the results of the study it can be concluded that ethanol extracts and fractions of nipah leaf fronds (Nypa fruticans.Wurmb) can inhibit the activity of xanthine oxidase enzyme with ethyl acetate fraction giving the highest inhibitory activity with IC50 value of 3,543±1,440 ppm.

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聂帕叶（nypa fruticans. wurmb）乙醇提取物和馏分的抗高血压活性

高尿酸血症是一种代谢综合征，其特点是血液中尿酸含量高。高尿酸血症晚期可引起痛风或热病（一种影响关节并导致关节损伤的疾病）、肾结石、肾损伤和高血压。尼帕植物（Nypa fruticans. Wurmb）传统上用于治疗胃痛、糖尿病、发烧、牙痛、降低尿酸和头痛。一般来说，聂帕植物的次生代谢化合物包括黄酮类、酚类、单宁、萜类、甾体、生物碱和皂苷。本研究的目的是测定聂帕叶（Nypa fruticans. Wurmb）乙醇提取物和馏分的黄嘌呤氧化酶抑制活性。萃取过程采用浸渍法，溶剂为 96% 的乙醇，分馏过程采用液-液分配法，分液漏斗。.使用紫外/可见分光光度计测试体外黄嘌呤氧化酶活性的抑制作用。结果表明，乙醇提取物、水馏分、乙酸乙酯馏分和正己烷馏分对黄嘌呤氧化酶具有抑制活性，其 IC50 值分别为 47.558 ± 2.862；18.755 ± 3.821；3.543 ± 1.440 和 110.213 ± 1.577 ppm。研究结果表明，聂帕叶（Nypa fruticans.Wurmb）的乙醇提取物和馏分可抑制黄嘌呤氧化酶的活性，其中乙酸乙酯馏分的抑制活性最高，IC50 值为 3,543±1,440 ppm。

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Proceeding of International Conference on Science, Health, And Technology

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