Biodegradation of aflatoxin B1 by Aspergillus terreus HNGD-TM15 and its degradation mechanism

IF 1.7 4区医学 Q3 FOOD SCIENCE & TECHNOLOGY

World Mycotoxin Journal Pub Date : 2023-10-12 DOI:10.1163/18750796-20222806

T. Wu, M. Ning, S. Zhang, Y. Xie, Q. Li, S. Sun

引用次数: 0

Abstract

The Aspergillus terreus strain HNGD-TM15 that was isolated from soil grown with rosemary was shown to efficiently degrade aflatoxin B1 (AFB1). Specifically, AFB1 was degraded by the strain’s fermentation broth at a maximum degradation rate of 98.3%. HPLC and LC-MS analyses detected a degradation product with an m/z ion value of 312.0636. Its molecular formula was C18H39NO3, which was tentatively identified as 2-amino-1, 3,4-octadecatriol. Based on LC-MS results and further analysis, it was revealed that a series of reactions, such as decomposition, reduction (lactone ring hydrogenation) and substitution (hydrolysis), occurred during the degradation of AFB1. Therefore, A. terreus HNGD-TM15 has a great potential for application in the detoxification of AFB1 contaminating food and feed products.

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土曲霉 HNGD-TM15 对黄曲霉毒素 B1 的生物降解及其降解机制

研究表明，从种植迷迭香的土壤中分离出的土曲霉菌株 HNGD-TM15 能有效降解黄曲霉毒素 B1（AFB1）。具体来说，该菌株的发酵液对 AFB1 的降解率最高可达 98.3%。HPLC 和 LC-MS 分析检测到一种降解产物，其 m/z 离子值为 312.0636。其分子式为 C18H39NO3，初步鉴定为 2-氨基-1，3，4-十八碳三醇。根据 LC-MS 结果和进一步分析，AFB1 降解过程中发生了分解、还原（内酯环氢化）和取代（水解）等一系列反应。因此，A. terreus HNGD-TM15 在对污染食品和饲料产品的 AFB1 进行解毒方面具有很大的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World Mycotoxin Journal MYCOLOGY-

CiteScore

4.60

自引率

5.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''World Mycotoxin Journal'' is a peer-reviewed scientific journal with only one specific area of focus: the promotion of the science of mycotoxins. The journal contains original research papers and critical reviews in all areas dealing with mycotoxins, together with opinions, a calendar of forthcoming mycotoxin-related events and book reviews. The journal takes a multidisciplinary approach, and it focuses on a broad spectrum of issues, including toxicology, risk assessment, worldwide occurrence, modelling and prediction of toxin formation, genomics, molecular biology for control of mycotoxigenic fungi, pre-and post-harvest prevention and control, sampling, analytical methodology and quality assurance, food technology, economics and regulatory issues. ''World Mycotoxin Journal'' is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as of policy makers and regulators.