DEVELOPMENT AND VALIDATION OF A NEW VISIBLE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF SOME ESSENTIAL OILS

IF 1.4 4区医学 Q4 PHARMACOLOGY & PHARMACY

FARMACIA Pub Date : 2023-10-26 DOI:10.31925/farmacia.2023.5.19

Ioana OLARIU

引用次数: 0

Abstract

This study aimed to develop and validate a visible spectrophotometry analysis method for fluconazole quantification in the presence of some essential oils (cinnamon, oregano and clove essential oil), by forming a coloured complex with Cu 2+ ion. For the spectrophotometric method validation the spectra of the fluconazole-Cu(II) complex in the presence of volatile oils were analysed and the wavelengths corresponding to the maximum absorbance values of the complex were determined. Evaluating the precision, linearity, accuracy and robustness of the elaborated visible spectrophotometric method, the obtained values of the specific parameters fell within the limits provided by the standards in force. Linearity was very good for the following fluconazole concentration ranges: 0.268 - 2.68 mg/mL for oregano essential oil, 0.2073 - 2.073 mg/mL for cinnamon essential oil and 0.2317 - 2.317 mg/mL in the case of clove essential oil. The proposed visible spectrophotometric method has been successfully applied for the assay of fluconazole in clove, cinnamon and oregano essential oils

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建立和验证测定某些精油中氟康唑的新可见分光光度法

本研究旨在开发和验证一种可见分光光度法分析方法，用于在一些精油（肉桂、牛至和丁香精油）存在的情况下，通过与 Cu 2+ 离子形成有色络合物，对氟康唑进行定量分析。为了验证分光光度法，分析了氟康唑-Cu(II) 复合物在挥发油存在下的光谱，并确定了与复合物最大吸光度值相对应的波长。在对所制定的可见分光光度法的精密度、线性度、准确度和稳健性进行评估时，所获得的特定参数值均在现行标准规定的范围内。在以下氟康唑浓度范围内，线性关系非常好：牛至精油为 0.268 - 2.68 mg/mL，肉桂精油为 0.2073 - 2.073 mg/mL，丁香精油为 0.2317 - 2.317 mg/mL。所提出的可见分光光度法已成功应用于丁香、肉桂和牛至精油中氟康唑的检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

FARMACIA 医学-药学

CiteScore

2.40

自引率

50.00%

发文量

审稿时长

6-12 weeks

期刊介绍： FARMACIA publishes original research papers, invited topical reviews and editorial commentaries and news, with emphasis on conceptual novelty and scientific quality. Main research areas are focused on: pharmacology, toxicology, medicinal chemistry, biopharmacy, drug design, drug delivery, personalized medicine, nanostructures, nutraceuticals, biochemistry and biotechnology. Manuscripts submitted to the Journal are only accepted after the peer review precess. The papers should have not been published in any other journal. The recommendations of the Declaration of Helsinki, for humans, and the International guidelines as accepted principles for the use of experimental animals should be followed.