Immunostimulant Compounds Identification in Indonesian Underutilized Zingiberaceae Spices

Q3 Chemistry

Molekul Pub Date : 2023-11-20 DOI:10.20884/1.jm.2023.18.3.7589

Dian Rosalina, N. D. Yuliana, Ririn Anggraeni, Endang Prangdimurti

{"title":"Immunostimulant Compounds Identification in Indonesian Underutilized Zingiberaceae Spices","authors":"Dian Rosalina, N. D. Yuliana, Ririn Anggraeni, Endang Prangdimurti","doi":"10.20884/1.jm.2023.18.3.7589","DOIUrl":null,"url":null,"abstract":"Spices are known for its health beneficial effect beside its function as flavouring and condiment. This study aimed to screen immunostimulant activities of 12 underutilized species of Zingiberaceae grown in Indonesia. The compound associated with immunostimulant activity in the most active sample was identified by linking the chromatogram profile of hexane, chloroform, ethyl acetate, methanol, and water fractions of the selected spices to its activity using orthogonal projection to the least square (OPLS) analysis, followed by LC-MS characterization. The result showed that Alpinia malaccensis and Zingiber ottensii had the highest lymphocyte cell proliferation index (SI) at concentrations of 400 ppm. A. malaccensis was chosen for the next step. The results of OPLS analysis showed that the retention time interval which strongly correlated with the immunostimulant activity was 27.0 – 28.5 minutes, which were abundant in methanol fraction. The mass spectra analysis of methanol fraction using UHPLC-HRMS showed that the respective retention time was attributed to flavokawain B. This compound was reported to increase rat splenocyte proliferation in vivo.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":"25 7","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molekul","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20884/1.jm.2023.18.3.7589","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Chemistry","Score":null,"Total":0}

引用次数: 0

Abstract

Spices are known for its health beneficial effect beside its function as flavouring and condiment. This study aimed to screen immunostimulant activities of 12 underutilized species of Zingiberaceae grown in Indonesia. The compound associated with immunostimulant activity in the most active sample was identified by linking the chromatogram profile of hexane, chloroform, ethyl acetate, methanol, and water fractions of the selected spices to its activity using orthogonal projection to the least square (OPLS) analysis, followed by LC-MS characterization. The result showed that Alpinia malaccensis and Zingiber ottensii had the highest lymphocyte cell proliferation index (SI) at concentrations of 400 ppm. A. malaccensis was chosen for the next step. The results of OPLS analysis showed that the retention time interval which strongly correlated with the immunostimulant activity was 27.0 – 28.5 minutes, which were abundant in methanol fraction. The mass spectra analysis of methanol fraction using UHPLC-HRMS showed that the respective retention time was attributed to flavokawain B. This compound was reported to increase rat splenocyte proliferation in vivo.

查看原文本刊更多论文

印度尼西亚未充分利用的姜科香料中的免疫刺激化合物鉴定

众所周知，香料除了具有调味和调味品的功能外，还具有有益健康的作用。本研究旨在筛选印度尼西亚生长的 12 种未充分利用的姜科植物的免疫刺激活性。利用正交投影最小二乘法（OPLS）分析，将所选香料的正己烷、氯仿、乙酸乙酯、甲醇和水馏分的色谱图谱与其活性联系起来，然后进行液相色谱-质谱表征，从而确定了最具活性样本中与免疫刺激活性相关的化合物。结果表明，在浓度为 400 ppm 时，Alpinia malaccensis 和 Zingiber ottensii 的淋巴细胞增殖指数（SI）最高。因此，下一步的研究选择了马缨丹。OPLS 分析结果表明，与免疫刺激活性密切相关的保留时间间隔为 27.0 - 28.5 分钟，甲醇馏分中含量丰富。利用 UHPLC-HRMS 对甲醇馏分进行的质谱分析表明，相应的保留时间归因于黄卡瓦胡椒素 B。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊