EVALUATION OF ANTI-TOXOPLASMA ANTIBODY DETECTION BY MODIFIED AGGLUTINATION TEST IN TISSUE OF EXPERIMENTALLY INFECTED MICE

Abstract

Toxoplasma gondii infection affects homoeothermic animals around the world, and many infections, in animals and man, can result in abortion, congenital disorders, ocular or neuromuscular disease, with serious impact in animal and public health. A variety of antibody detection methods have been proposed since initial description of Sabin-Feldman dye test, usually using blood sera as sample for detection. Tissues and fetal liquids can be used for antibody detection too, but few research compare the performance of this detection in pairwise sera, and the evaluation of autolysis effect was not been verified. This work shows the results of sensibility, specificity, and the efficiency of direct agglutination method for anti-T. gondii antibody detection in fresh or autolysed samples of liver, spleen, and muscle, obtained of chronically infected mice.