Isolation of bacteria producing lipase from copra (Cocos nucifera l.) Cake for degradation of vegetable waste oils

Abstract

Vegetable waste oil consists of long chain triglycerides (LCTs). Waste oil from the kitchen causes clogging of the pipe as they solidify while passing from the kitchen. The waste with oil in the garbage will be drained into the ground and then into ground water which will lead to pollution of the ground water. Lipase is an enzyme used to break down the triglycerides into free fatty acids and glycerol by catalyzing the hydrolysis of the ester bonds in triglycerides. Bacteria Lipase is isolated from copra (Cocos nucifera L.) cake. The isolated bacteria were identified by the biochemical characterization and was sequenced by 16sRNA. The lipase production by the bacteria was identified by tween 80. The enzyme activity was identified by titrimetric method. Enzyme was extracted by salting out method and was partially purified by the SDS – PAGE.