Determination of in vitro thrombolytic activity in isolated flavonoids of plant Madras Nelli

Raj Padhya Ramesh, Maharjan Sajan, Khanal Amrit, Pokhrel Thakur, Krishna Burdipad Gopala
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Abstract

The study is regarded for standardization of aerial parts of the plant Phyllanthus maderaspatensis L., phytochemical investigations, isolation of flavonoids and evaluation for thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract. Standardization study includes macroscopy, microscopy and determination of proximate values and phytochemical investigations include extraction of coarsely powdered herb with exhaustive alcoholic and aqueous extractions and successive extraction with petroleum ether, chloroform and alcohol. The extracts were subjected to qualitative chemical analysis, chromatographic studies [Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC)] were performed for flavonoids detection in alcoholic extract. Isolated two flavonoids from butanolic fraction of alcoholic extract by column chromatography and preparative TLC, their partial characterization was done with Proton Nuclear Magnetic Resonance (PNMR) and Fourrier Transform Infrared (FT-IR) spectral data. Thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract (20 and 10 mg/100 µl) were evaluated in incubated blood. An attempt made to standardize aerial parts of P. maderaspatensis L., was successful. Two flavonoids were isolated; the alcoholic extract showed significant thrombolytic effect in comparison with thrombosis control in comparison with standard thrombolytic agent streptokinase (30000 unit/100 µl).
测定马德拉斯内利植物中分离出的黄酮类化合物的体外溶栓活性
该研究旨在对植物 Phyllanthus maderaspatensis L. 的气生部分进行标准化、植物化学研究、黄酮类化合物的分离以及酒精提取物和酒精提取物丁醇部分的溶栓活性评估。标准化研究包括宏观镜检、显微镜检和近似值测定,而植物化学研究则包括对粗粉末药草进行提取,包括彻底的酒精提取和水提取,以及石油醚、氯仿和酒精的连续提取。对提取物进行了定性化学分析,并对酒精提取物中的黄酮类化合物进行了色谱分析(薄层色谱法(TLC)和高效薄层色谱法(HPTLC))。通过柱层析和制备型 TLC 从酒精提取物的丁醇馏分中分离出两种黄酮类化合物,并利用质子核磁共振(PNMR)和傅立叶变换红外光谱(FT-IR)数据对其进行了部分表征。在培养血液中评估了酒精提取物和酒精提取物丁醇部分(20 和 10 mg/100 µl)的溶栓活性。对 P. maderaspatensis L. 的气生部分进行标准化的尝试取得了成功。分离出了两种黄酮类化合物;酒精提取物与标准溶血剂链激酶(30000 单位/100 µl)相比,具有明显的溶栓效果。
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