Analysis of Curcumin Contents in Curcuma xanthorrhiza using FTIR Spectroscopy and HPLC-UV in Combination with Multivariate Calibration

Majalah Obat Tradisional Pub Date : 2023-11-30 DOI:10.22146/mot.82220

A. P. Kusumadewi, Rohmat Mujahid, Ronny Martien, Purwanto Purwanto, A. A. Styawan, I. Irnawati, Abdul Rohman

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引用次数: 0

Abstract

Curcuma xanthorrhiza Roxb. or Java Turmeric with the local name of Temulawak is one of herbal medicines used in Indonesia. This plant is believed to prevent some degenerative diseases due to its active compounds, especially curcumin (CUR) contained in Java Turmeric. Therefore, analysis of active components including CUR is very urgent. This study highlighted the development of FTIR spectroscopy coupled with PLSR for the determination of CUR in Java Turmeric powders. The levels of CUR in Java Turmeric powders were determined using HPLC with UV detectors, and the obtained results were used as actual values to be predicted using FTIR spectroscopy-multivariate calibrations. The results revealed that the levels of CUR ranged from 0.6741 ± 0.0705% (g/100 samples) to 2.1062 ± 0.0095%. PLSR modeling for the relationship between the actual value of CUR as determined using HPLC and calculated values as predicted using FTIR spectroscopy provide the value of R2 of 0.9990 with RMSEC of 0.0028. The developed method offers reliable results providing a green analytical method due to the use of minimum solvent and reagent and does not involve extensive sample preparation.

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利用傅立叶变换红外光谱和高效液相色谱-紫外光谱结合多元校准分析莪术中的姜黄素含量

姜黄（Curcuma xanthorrhiza Roxb.）或爪哇姜黄（当地名称为 Temulawak）是印度尼西亚使用的草药之一。这种植物被认为能预防一些退化性疾病，因为它含有活性化合物，尤其是爪哇姜黄中的姜黄素（CUR）。因此，对包括姜黄素在内的活性成分进行分析非常迫切。本研究重点开发了傅立叶变换红外光谱与 PLSR 联用技术，用于测定爪哇姜黄粉中的姜黄素。使用带有紫外检测器的高效液相色谱法测定了爪哇姜黄粉中的 CUR 含量，并使用傅立叶变换红外光谱多变量定标法将所得结果作为实际值进行预测。结果显示，CUR 的含量范围为 0.6741 ± 0.0705%（克/100 个样品）至 2.1062 ± 0.0095%。对使用高效液相色谱法测定的 CUR 实际值和使用傅立叶变换红外光谱法预测的计算值之间的关系进行 PLSR 建模，结果表明 R2 值为 0.9990，RMSEC 为 0.0028。所开发的方法使用的溶剂和试剂最少，无需进行大量的样品制备，因此结果可靠，是一种绿色分析方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Majalah Obat Tradisional

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

15 weeks