Ethanolic extract of Aloe arborescens stimulates neonatal rat calvarial cells proliferation, migration and osteogenic differentiation

Q3 Agricultural and Biological Sciences
Nicolás Olegario Blanco, Valeria Gili, J. Laiuppa, Graciela Edith Santillán
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引用次数: 0

Abstract

The medicinal plant Aloe arborescens Miller has chemical compounds that could stimulate the activity of bone-forming cells, but no studies have been found in this regard. We evaluated the effects of different dilutions of aqueous (1/10; 1/100; 1/1000) or ethanolic (1/1000; 1/2000; 1/5000) extracts of parenchyma from A. arborescens on the viability, proliferation, migration and osteogenic differentiation of primary cell cultures from neonatal rat calvaria. In none of the conditions studied did cell viability decrease (p ≤ 0.01). Furthermore, the 1/5000 ethanolic extract dilution showed a positive effect on cell viability at 48 h and 72 h and the latter was correlated with a 27 % (p ≤ 0.01) increase in cell proliferation. Ethanolic extract significantly stimulated cell migration and cultured mineralization with respect to control, showing the maximal effect at a dilution 1/5000. Together, the results show that the A. arborescens extracts do not have toxic effects. In addition, ethanolic extract stimulates proliferation, migration and osteogenic differentiation of rat calvarial cells, suggesting a potential bone anabolic action.
芦荟乙醇提取物可刺激新生大鼠钙化细胞增殖、迁移和成骨分化
药用植物芦荟(Aloe arborescens Miller)含有可刺激骨形成细胞活性的化学物质,但尚未发现这方面的研究。我们评估了不同稀释度的芦荟实质水提取物(1/10;1/100;1/1000)或乙醇提取物(1/1000;1/2000;1/5000)对新生大鼠小腿原代细胞培养物的活力、增殖、迁移和成骨分化的影响。在所有研究条件下,细胞活力均未下降(p ≤ 0.01)。此外,1/5000 乙醇提取物稀释液在 48 小时和 72 小时内对细胞存活率有积极影响,后者与细胞增殖增加 27 %(p ≤ 0.01)相关。与对照组相比,乙醇提取物能明显促进细胞迁移和培养矿化,在稀释度为 1/5000 时效果最大。这些结果表明,A. arborescens 提取物没有毒性作用。此外,乙醇提取物还能刺激大鼠腓肠肌细胞的增殖、迁移和成骨分化,表明其具有潜在的骨合成作用。
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来源期刊
Biological Communications
Biological Communications Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.70
自引率
0.00%
发文量
21
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