The preparation of samples for studying neutrophils without their isolation

Abstract

Neutrophils are the primary cells of the innate immune system. They destroy pathogenic microorganisms carrying out the oxidative stress mechanism through phagocytosis and extracellular traps (NETs). There is no doubt about the need to study neutrophils’ functional activity, but there are some methodological problems with the nativity of conditions in such experiments. The aim of this work is to propose a method of preparing a sample of peripheral blood neutrophils for study by spectral-optical methods (such as confocal microscopy) providing an increased level of the nativity of the conditions.