SOME ASPECTS OF CREATION OF HEALTHY GRAPEVINE COLLECTIONS OF ABORIGINAL DON VARIETIES IN VITRO

Abstract

The article discusses some aspects of improving the elements of technology for creating of healthy collection of indigenous grape varieties in vitro. To recover from phytoplasma infec-tion, a chemotherapy method using the antibi-otic Cefotaxime has been developed. At high concentrations of the antibiotic, intoxication of plants and deterioration in their quality charac-teristics were noted. Plants of Makhrovatchik variety showed an improvement in survival rate at antibiotic concentrations of 100 and 200 mg/l: 64.3 and 60.7%, respectively. For long-term cultivation without replantings, the effectiveness of slowing down growth process-es by increasing the density of the nutrient me-dium was studied. The study was carried out on the varieties Makhrovatchik, Tsimlyansky Sergienko. Studied concentrations of agar-agar were (6, 8, 10, 12, 13 and 15 g/l). A decrease in the rate of growth processes was noted in all studied varieties, but the rate of the decrease was different.