Effects of Acetaminophen on the Reproductive Parameters and the Ameliorative Effects of Rutin and Mesenchymal Stem Cells in Male Rats

The Scientific Journal of University of Benghazi Pub Date : 2023-12-06 DOI:10.37376/sjuob.v36i2.4301

Rabihah E. A. Elduob, Eda M. A. Alshailabi, Samia M. Efkeren

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Abstract

The present study has been designed to evaluate the effects of acetaminophen on the reproductive parameters and the ameliorative effects of rutin and Mesenchymal stem cells in male rats. The rats were divided into two studies: firstly, twenty young male rats were used as a source of bone marrow-derived MSCs. Secondly, fifty adult male rats were divided into 5 groups: Control group (G1); rats were administrated of AAP (750 mg/kg b.w.) every 72h for 21 days, then left for 30 and 60 days without treatment (G2), rats were administrated of AAP for 21 days then treated with RT (25mg/kg b.w/d) for 30 and 60 days (G3), rats were administrated of AAP for 21 days then the rats were injected by BM-MSCs (1.5x 106 cells in 0.5 PBS) in the tail vein for 30 and 60 days (G4), and rats were administrated of AAP for 21 days then the rats were injected by BM-MSCs in the tail vein, then treated with RT for 30 and 60 days (G5). Administration of AAP produced a significant decrease in the mean value of the Te, LH, and FSH levels after 30 and 60 days as compared to the C groups. Whereas rats treated with RT and MSCs showed a significantly high increase in the mean values of these hormone levels after 30 and 60 days compared with the AAP group. In conclusion, this study demonstrated that RT and MSCs when treated in combination, are protected against the AAP-induced decrease in the hormone level of testicular.

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对乙酰氨基酚对雄性大鼠生殖参数的影响以及芦丁和间充质干细胞的改善作用

本研究旨在评估对乙酰氨基酚对雄性大鼠生殖参数的影响以及芦丁和间充质干细胞的改善作用。大鼠分为两项研究：首先，20 只年轻雄性大鼠被用作骨髓间充质干细胞的来源。其次，将 50 只成年雄性大鼠分为 5 组：对照组（G1）；每 72 小时给大鼠注射一次 AAP（750 毫克/千克体重），连续 21 天，然后不做任何处理，分别放置 30 天和 60 天（G2）；给大鼠注射 AAP 21 天，然后用 RT（25 毫克/千克体重/天）治疗 30 天和 60 天（G3）。给大鼠注射抗逆转录病毒药物 21 天，然后用 RT（25 毫克/千克 b.w/d）治疗 30 天和 60 天（G3）；给大鼠注射抗逆转录病毒药物 21 天，然后在尾静脉注射 BM-间充质干细胞（1.5x 106 cells in 0.5 PBS），治疗 30 天和 60 天（G4）；给大鼠注射抗逆转录病毒药物 21 天，然后在尾静脉注射 BM-间充质干细胞，再用 RT 治疗 30 天和 60 天（G5）。与 C 组相比，给予 AAP 30 天和 60 天后，Te、LH 和 FSH 水平的平均值明显下降。而接受 RT 和间充质干细胞治疗的大鼠与 AAP 组相比，在 30 天和 60 天后，这些激素水平的平均值明显增加。总之，这项研究表明，RT 和间充质干细胞联合治疗可防止 AAP 引起的睾丸激素水平下降。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Scientific Journal of University of Benghazi

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