New Record of Scenedesmus Vacuolatus From Soil In Vojvodina, Serbia

IF 0.6 4区生物学 Q4 PLANT SCIENCES

Bangladesh Journal of Plant Taxonomy Pub Date : 2023-12-24 DOI:10.3329/bjpt.v30i2.70501

T. H. Jafari, Vladimira Žunić, S. Djuric, Dragana Stamenov

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引用次数: 0

Abstract

Soil algae are photosynthetic microorganisms showing huge differences in morphology depending on the soil type, agricultural practices, and environmental factors. The aim of this research was to compare the results of classical and molecular protocols for microalgal identification. The green microalgae were isolated from acidic arable soil of dystric cambisol type, using BG11 medium and the agar plate method. Firstly, the identification of the microalgae was performed based on its morphological characteristics using light microscopy (LM) and taxonomic monographs and reference books. The isolate was initially annotated on genus level as Coelastrella sp. Gene marker (ITS2) was used for molecular identification. According to this protocol, the microalga was identified as Scenedesmus vacuolatus. Combination of morphology and DNA-based approach proved to be the most effective for obtaining a consistent species level identification. Moreover, this is the first record of Scenedesmus vacuolatus identification from soil in Vojvodina, Serbia. Bangladesh J. Plant Taxon. 30(2): 255-261, 2023 (December)

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塞尔维亚伏伊伏丁那省土壤中的新记录 Scenedesmus Vacuolatus

土壤藻类是一种光合微生物，其形态因土壤类型、农业实践和环境因素的不同而存在巨大差异。本研究旨在比较微藻鉴定的传统方法和分子方法的结果。研究人员使用 BG11 培养基和琼脂平板法，从酸性可耕地土壤中分离出绿色微藻。首先，使用光学显微镜（LM）和分类专著及参考书，根据微藻的形态特征对其进行鉴定。分离的微藻被初步标注为 Coelastrella sp.，并使用基因标记（ITS2）进行分子鉴定。根据这一方法，该微藻被鉴定为 Scenedesmus vacuolatus。结合形态学和基于 DNA 的方法被证明是获得一致的物种鉴定的最有效方法。此外，这是首次从塞尔维亚 Vojvodina 的土壤中鉴定出 Scenedesmus vacuolatus。孟加拉国植物分类学杂志30(2):255-261，2023 年（12 月）

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来源期刊

Bangladesh Journal of Plant Taxonomy 生物-植物科学

CiteScore

0.42

自引率

44.40%

发文量

审稿时长

6 months

期刊介绍： Bangladesh is a humid, subtropical country favouring luxuriant growth of microorganisms, fungi and plants from algae to angiosperms with rich diversity. She has the largest mangrove forest of the world in addition to diverse hilly and wetland habitats. More than a century back, foreign explorers endeavoured several floral expeditions, but little was done for non-vasculars and pteridophytes. In recent times, Bangladesh National Herbarium has been carrying out taxonomic research in Bangladesh along with few other national institutes (e.g. Department of Botany of public universities and Bangladesh Forest Research Institute).