Influence of 60-day administration of tartrazine and infliction of a defect in the tibia on the histological structure and morphometric parameters of the rat’s thyroid gland

V. N. Morozov, V. I. Luzin
{"title":"Influence of 60-day administration of tartrazine and infliction of a defect in the tibia on the histological structure and morphometric parameters of the rat’s thyroid gland","authors":"V. N. Morozov, V. I. Luzin","doi":"10.17021/1992-6499-2023-4-58-66","DOIUrl":null,"url":null,"abstract":"Aim: to study the histological structure and histomorphometric parameters of the thyroid gland in rats in the period after the completion of the 60-day administration of tartrazine and the creation of a through defect in the tibiae.Material and methods. One hundred and fifty white male rats were divided into five groups: groups T1 and T2 - 60 days of exposure to tartrazine at a dosage of 750 and 1500 mg/kg; group K+D – 60-day administration of saline solution and infliction of a defect in the tibiae; groups T1+D and T2+D – 60-day exposure to tartrazine at a dosage of 750 and 1500 mg/kg and causing a defect in the tibia. The histological structure and histomorphometric parameters of the thyroid gland were studied on days 3, 10, 15, 24 and 45.Results. In the T1+D and T2+D groups, mainly small follicles, lined with cuboidal shaped cells, and in some of them - a flat or columnar shaped cells were detected in the center of the gland from days 3 to 15. Many thyrocytes had a vacuolated cytoplasm and an intensely stained nucleus. The heterogeneous colloid partially filled or was practically absent in the follicles cavity, anemia of the capillaries between them was determined. Singly areas of the organ parenchyma with a significant accumulation of lymphocytes were detected. Histomorphometric study showed a decrease in the internal diameter of the follicles, lumen-epithelial index and nucleo-cytoplasmic ratio and the raise in the height of the follicular epithelium and the area of the cytoplasm of follicular cells.Conclusion. Sixty-day administration of tartrazine to rats and artificial traumatic bone injury leads, in comparison with the data of control groups, to a more pronounced change in the histological picture and morphometric data of the thyroid gland. The intensity and duration of changes are dosedependent: in the T1+D group, most of the qualitative and quantitative changes are smoothed out by 45 days, and in the T2+D group, many of them remain, and the body does not adapt to them.","PeriodicalId":269283,"journal":{"name":"ASTRAKHAN MEDICAL JOURNAL","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ASTRAKHAN MEDICAL JOURNAL","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17021/1992-6499-2023-4-58-66","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Aim: to study the histological structure and histomorphometric parameters of the thyroid gland in rats in the period after the completion of the 60-day administration of tartrazine and the creation of a through defect in the tibiae.Material and methods. One hundred and fifty white male rats were divided into five groups: groups T1 and T2 - 60 days of exposure to tartrazine at a dosage of 750 and 1500 mg/kg; group K+D – 60-day administration of saline solution and infliction of a defect in the tibiae; groups T1+D and T2+D – 60-day exposure to tartrazine at a dosage of 750 and 1500 mg/kg and causing a defect in the tibia. The histological structure and histomorphometric parameters of the thyroid gland were studied on days 3, 10, 15, 24 and 45.Results. In the T1+D and T2+D groups, mainly small follicles, lined with cuboidal shaped cells, and in some of them - a flat or columnar shaped cells were detected in the center of the gland from days 3 to 15. Many thyrocytes had a vacuolated cytoplasm and an intensely stained nucleus. The heterogeneous colloid partially filled or was practically absent in the follicles cavity, anemia of the capillaries between them was determined. Singly areas of the organ parenchyma with a significant accumulation of lymphocytes were detected. Histomorphometric study showed a decrease in the internal diameter of the follicles, lumen-epithelial index and nucleo-cytoplasmic ratio and the raise in the height of the follicular epithelium and the area of the cytoplasm of follicular cells.Conclusion. Sixty-day administration of tartrazine to rats and artificial traumatic bone injury leads, in comparison with the data of control groups, to a more pronounced change in the histological picture and morphometric data of the thyroid gland. The intensity and duration of changes are dosedependent: in the T1+D group, most of the qualitative and quantitative changes are smoothed out by 45 days, and in the T2+D group, many of them remain, and the body does not adapt to them.
对大鼠甲状腺组织学结构和形态计量参数施加60天的酒石酸并造成胫骨缺损的影响
目的:研究大鼠在服用 60 天的酒石酸并在胫骨上造成贯通缺损后,甲状腺的组织学结构和组织形态学参数。150只白色雄性大鼠被分为五组:T1组和T2组--暴露于酒石酸60天,剂量分别为750毫克/千克和1500毫克/千克;K+D组--服用生理盐水60天,并造成胫骨缺损;T1+D组和T2+D组--暴露于酒石酸60天,剂量分别为750毫克/千克和1500毫克/千克,并造成胫骨缺损。在第3、10、15、24和45天对甲状腺的组织学结构和组织形态计量参数进行了研究。在T1+D组和T2+D组中,从第3天到第15天,腺体中心发现的主要是小滤泡,内衬是立方体状细胞,其中一些是扁平或柱状细胞。许多甲状腺细胞的细胞质呈空泡状,细胞核被浓染。滤泡腔内部分充满或几乎没有异质胶质,滤泡间的毛细血管贫血。在器官实质的单个区域检测到大量淋巴细胞聚集。组织形态计量学研究显示,卵泡内径、管腔上皮指数和细胞核-细胞质比率下降,卵泡上皮高度和卵泡细胞的细胞质面积增加。与对照组的数据相比,给大鼠服用60天的酒石酸和人工创伤性骨损伤会导致甲状腺的组织学图像和形态计量学数据发生更明显的变化。变化的强度和持续时间与剂量有关:在T1+D组中,大多数质和量的变化在45天后都已平滑消失,而在T2+D组中,许多变化仍然存在,机体无法适应这些变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信