EVALUASI PEMERIKSAAN BILIRUBIN TOTAL MENGGUNAKAN MODEL SPECIMEN INSIGNIFICANT DAN MILD HEMOLYSIS

GEMA KESEHATAN Pub Date : 2023-12-28 DOI:10.47539/gk.v15i2.428

S. Kesuma, Siti Rodiyah, Maulida

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引用次数: 0

Abstract

Bilirubin total merupakan salah satu pemeriksaan fungsi hati. Pemeriksaan bilirubin total umumnya menggunakan sampel darah yang tidak boleh hemolisis, sehingga sampel hemolisis menjadi salah satu faktor yang dapat mempengaruhi hasil pemeriksaan bilirubin total. Hemolisis dapat terjadi secara in vivo dan in vitro. Hemolisis secara in vitro menyebabkan kesalahan pemeriksaan bilirubin total. Tujuan penelitian ini untuk mengetahui hasil evaluasi pemeriksaan bilirubin total menggunakan model specimen insignificant dan mild hemolysis. Metode penelitian ini adalah kuasi eksperimen. Pembuatan hemolisat menggunakan larutan aquadest. Jumlah sampel pada penelitian ini adalah 40 sampel. Pemeriksaan menggunakan metode kolorimetri pada alat fotometer DIRUI DR-7000D. Pemeriksaan hemoglobin digunakan untuk melakukan validasi specimen insignificant dan mild hemolysis. Hasil uji statistik one-way Anova didapatkan nilai Sig < 0.05. Hasil akurasi yang didapatkan adalah -1.83% dan -4.40% < 11.4%, presisi 22.0% dan 20.6% > 7%, TE 38.1% dan 38.5% > 20% TEa berturut-turut sampel insignificant dan mild hemolysis. Kesimpulan terdapat perbedaan secara statistik dan klinis pada hasil evaluasi pemeriksaan bilirubin total model specimen insignificant dan mild hemolysis. Kata kunci: Bilirubin Total, Specimen Insignificant Hemolysis, Specimen Mild Hemolysis The total bilirubin is one of the liver function tests. The entire bilirubin test generally uses blood samples, which are one of the factors that can’t be hemolyzed, so hemolysis samples are one of the factors that can affect the result of the total bilirubin test. Hemolysis can occur in vivo and in vitro. Hemolysis in vitro causes an error in the entire bilirubin test. However, hemolysis specimens are still examined for total bilirubin under certain conditions. The evaluation aims to find out the results of natural bilirubin test specimens insignificant and mild hemolysis. This research method is quasi-experimental. They are making hemolysate using an aquadest solution. The number of samples in this research was 40, and examination was done using the colorimetric method on the DIRUI DR-7000D photometer. A hemoglobin test is used to validate specimens of insignificant and mild hemolysis. The one-way Anova statistical test results obtained a sig value < 0.05. Accuracy results were -1.83% and -4.40% < 11.4%, precision 22.0% and 20.6% > 7%, TE 38.1% and 38.5% > 20% TEa, respectively, samples of insignificant and mild hemolysis. The conclusion: statistical and clinical differences exist in evaluating total bilirubin test results in the tiny and soft hemolysis specimen models. Keywords: Insignificant Hemolysis Specimen, Mild Hemolysis Specimen, Total Bilirubin

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使用微量样本和轻度溶血模型对总胆红素测定进行评估

总胆红素是最重要的肝脏疾病诊断指标。总胆红素可产生血红素沉积，而血红素沉积则是最有效的总胆红素沉积因子。体内和体外血红蛋白都有可能发生。体外溶血可提高胆红素总量。本实验用于评估轻微溶血和微量溶血模型样本的总胆红素。其评估方法为样本分析。血红蛋白可检测水样幼虫。该疗法的样本量为 40 个样本。使用 DIRUI DR-7000D 型照相测色仪进行测量。血红蛋白测量用于验证不明显和轻微溶血样本。单因素方差分析的统计结果显示，Sig < 0.05 为零。结果显示，-1.83% 和 -4.40% < 11.4%、22.0% 和 20.6% > 7%、38.1% 和 38.5% > 20% TEa 的样本溶血不明显，且为轻度溶血。统计和临床数据可用于评估不显著和轻度溶血样本的胆红素总量。Kata kunci: 总胆红素、不显著溶血标本、轻度溶血标本总胆红素是肝功能检测项目之一。总胆红素检测一般使用血液样本，而血液样本是不能溶解的因素之一，因此溶血样本是影响总胆红素检测结果的因素之一。溶血可发生在体内和体外。体外溶血会导致整个胆红素检测结果出现误差。然而，在某些条件下，溶血标本仍可检测总胆红素。评估的目的是找出自然胆红素试验标本微量和轻度溶血的结果。本研究采用准实验法。他们使用含水溶液制作溶血液。本次研究的样本数量为 40 个，使用 DIRUI DR-7000D 光度计的比色法进行检测。血红蛋白测试用于验证溶血不明显和轻微的样本。单向 Anova 统计检验结果显示，sig 值小于 0.05。不显著和轻度溶血样本的准确度结果分别为-1.83%和-4.40% < 11.4%，精确度分别为22.0%和20.6% > 7%，TE分别为38.1%和38.5% > 20% TEa。结论：在微小溶血和轻度溶血标本模型中评估总胆红素检测结果存在统计学和临床差异。关键词不显著溶血标本、轻度溶血标本、总胆红素

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