Fungal endophytic species Fusariumannulatum and Fusariumsolani: identification, molecular characterization, and study of plant growth promotion properties

Abstract

Research on endophytic fungi has gained significant interest due to their potential to enhance plant growth directly by producingphytohormones, solubilizing macronutrients, fixing nitrogen, or indirectly inhibiting phytopathogens growth by producing ammonia, siderophore, hydrogen cyanide, or extracellular enzymes, thereby acting as biocontrol agents. The present study aimed to isolate fungal endophytes from Alternantheraphiloxeroidesand evaluate their plant growth promotion and antimicrobial activity. In total, nine fungal endophytic strains were isolated from different parts of A. philoxeroides such as leaves, roots, and stems. The results demonstrate that the strains MEFAphS1 and MEFAphR3 exhibited positive plant growth promotion properties,including phosphate solubilization, and IAA (Indoleacetic acid) production, and ammonia production. The IAA production was highest for MEFAphS1, with a concentration of 46.635±1.04 µg/mL, while MEFAphR3 displayed the highest ammonia production (0.903±0.01 µg/mL). The phosphate solubilization index (PSI) is the maximum for MEFAphS1 (1.5±0.10). MEFAphS1 also exhibited antibacterial activity against Vibrio vulnificus, Streptococcus pneumoniae, and V.parahaemolyticus,with the most substantial inhibition zone observed against V.vulnificus(28±1 mm). In contrast, MEFAphR3 showed an inhibition zone of 8±1.53 mm against V. parahaemolyticus. Molecular identification revealed the identity of the isolates MEFAphS1 and MEFAphR3 as Fusariumsolaniand F.annulatum. These results thus confirm the possible applications of the fungal endophytes as plant biofertilizers and bio-enhancers to increase crop productivity.