The Characterization of Volatile Compounds of Lupin Türkiye Genotype HS-SPME/GC-MS Method

E. N. SIMSEK SEZER, Mustafa Yorgancilar, Tuna Uysal
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Abstract

The aim of this study is to reveal the volatile and semi-volatile constituent of the local Lupinus albus L. genotype grown in Türkiye by using the HS-SPME technique. Local lupin is a legume plant with great potential due to its high seed yield and protein and oil content in the seeds. Powdered seeds of local lupin genotypes were analysed and compared with respect to types and contents of volatile semi-volatile compounds contents using four different SPME fiber with GC-MS system in W9N11, SWGDR4G4 and SWGDR4G5 libraries. Fiber with 50/30 µm DVB/CAR/PDMS identified 54 compounds, 65 µm PDMS/DVB fiber 97 compounds, 85 µm Carboxen/PDMS fiber 28 compounds, and 85 µm polyacrylate 12 compounds. As a result, 65 µm PDMS/DVB fiber is the most preferable in terms of compound identification, with 97 compound. In terms of content, Benzene, Methyl(1-Methylethyl) (39.92%), Gamma-Terpinene (12.26%), Cis-Ocimene (5.93%), 1,3,6-Octatriene,3,7-Dimethyl-(% 5.51), Beta-Myrcene (4.70%), Alpha-Pinene (4.39%), Alpha.-Thujene (4.21%), Alpha Terpinene (2.55%), Camphene (2.23%), 1,6-Octadien-3-Ol,3,7-Dimethyl (2.10%) were mostly detected in the analysis with 50/30 µm DVB/CAR/PDMS fiber. With the other fiber 65 µm PDMS/DVB; phospine oxide, triphenyl- (15.49 %), pulegone (5.03 %), L-linalool (3.85 %), cyclopentasiloxane, decamethyl (3.54 %), cyclohexasiloxane, dodecamethyl (3.13 %), cyclotrisiloxane, hexamethyl (2.32 %), oxime-,methoxy-phenyl (2.08 %), 1-pentanamine,N-pentyl (1.60 %), 3,5-octadıen-2-one (1.55 %), I-menthone (1.50 %). Fiber 85 µm Carboxen/PDMS; the most four components in the content are; Benzene, 2, 4-diisocyanato-1-methyl (54.27 %), Pyrrole-3-carbonitrile, 5-formyl-2, 4 (18.41 %), 1-Butanol, 4-(1-methylethoxy) (14.67 %), pyridine,4-(1-pyrrolidinyl)- (8.13 %). With 85 µm polyacrylate fibre the most found component is 1, 2-Benzenedicarboxylic acid, diethyl ester (98.90 %). In conclusion, as far as we know this study is the first study about the volatile content of local lupin genotype of Türkiye.
图尔基耶羽扇豆基因型挥发性化合物的表征 HS-SPME/GC-MS 方法
本研究的目的是利用 HS-SPME 技术揭示生长在土耳其的当地羽扇豆基因型的挥发性和半挥发性成分。本地羽扇豆是一种豆科植物,因其种子产量高、蛋白质和油脂含量高而具有巨大潜力。在 W9N11、SWGDR4G4 和 SWGDR4G5 库中,使用四种不同的 SPME 纤维和 GC-MS 系统分析和比较了当地羽扇豆基因型的粉末种子中挥发性半挥发性化合物的种类和含量。使用 50/30 µm DVB/CAR/PDMS 的纤维鉴定出 54 种化合物,使用 65 µm PDMS/DVB 的纤维鉴定出 97 种化合物,使用 85 µm Carboxen/PDMS 的纤维鉴定出 28 种化合物,使用 85 µm 聚丙烯酸酯的纤维鉴定出 12 种化合物。因此,就化合物鉴定而言,65 微米 PDMS/DVB 纤维最适合鉴定 97 种化合物。从含量上看,苯,甲基(1-甲基乙基)(39.92%),γ-萜品烯(12.26%),顺式-茴香烯(5.93%),1,3,6-辛三烯,3,7-二甲基-(5.51%),β-月桂烯(4.70%),α-蒎烯(4.39%),α.-�烯(4.21%),α.-蒎烯(4.39%),α.-�烯(4.39%)。在使用 50/30 µm DVB/CAR/PDMS 光纤进行的分析中,主要检测到了 β-月桂烯(4.70%)、α-蒎烯(4.39%)、α.-��烯(4.21%)、α-萜品烯(2.55%)、莰烯(2.23%)、1,6-辛二烯-3-Ol,3,7-二甲基(2.10%)。使用另一种 65 µm PDMS/DVB 纤维时;氧化磷,三苯基(15.49 %),桉醛(5.03 %),L-芳樟醇(3.85 %),环五硅氧烷,十甲基(3.54 %),环己硅氧烷,十二甲基(3.13 %),环三硅氧烷,六甲基(2.32 %),肟,甲氧基苯基(2.08 %),1-戊胺,N-戊基(1.60 %),3,5-辛二烯-2-酮(1.55 %),I-薄荷酮(1.50 %)。纤维 85 µm Carboxen/PDMS;含量最多的四种成分是:苯,2,4-二异氰酸基-1-甲基(54.27 %),吡咯-3-甲腈,5-醛基-2,4(18.41 %),1-丁醇,4-(1-甲基乙氧基)(14.67 %),吡啶,4-(1-吡咯烷基)-(8.13 %)。在 85 微米的聚丙烯酸酯纤维中,发现最多的成分是 1,2-苯二甲酸二乙酯(98.90%)。总之,据我们所知,这项研究是首次对土耳其当地羽扇豆基因型的挥发性成分进行研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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