Study of the cytotoxic activity of alcoholic and aqueous extracts of Iraqi aromatic rice bran on B16 cell line

Abstract

The test was conducted on a sample consisting of mixing three samples of Iraqi Aromatic rice (Amber) bran obtained from the private mills in Najaf Governorate. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method was used to study the cytotoxic effect of alcoholic and aqueous extract of Iraqi Aromatic rice bran on B16 cell line, it is a murine tumor cell line used as a model to study human melanoma. The results showed that all concentrations of alcoholic extract (5, 10, 20, 40, and 80) % caused a significant inhibition on the B16 cell line. The inhibition rate reached to (13.00, 27.00, 47.33, 71.67 and 86.67) % respectively, after 48 hours while the cell viability was (87.00, 73.00, 52.67, 28.33and 13.33) % with a statistically significant difference at P≤. 0.05 compared to the control. The results showed that the aqueous extract had less inhibition effectiveness (for the same concentrations) after 48 hours, (10.00, 19.33, 27.00, 46.67 and 66.33) % and the cell viability rate was (90.00, 80.67, 73.00, 53.33 and 33.67) % respectively. The study shows that both alcoholic and aqueous extract prepared from Iraqi Aromatic rice bran had a toxic and anti-proliferative effect on B16 cell line, and this effect increases with increasing concentration. Treatment of these cells with the two extracts caused shrinkage, a decrease in numbers, and a clear morphological change in this type of cells.