Effect of diluters on frozen semen production of Black Bengal Goat

Bangladesh Journal of Animal Science Pub Date : 2023-12-31 DOI:10.3329/bjas.v54i4.70700

MA Rimi, T. Akter, MM Akhtar, M. Khandoker

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Abstract

The present study was undertaken to investigate the efficacy of three diluters (Triladyl, Andromed and Tris) for the production of frozen semen in Black Bengal goat and their effect on conception rate. Over the course of 6 months, data from six bucks were collected. Each collected sample was diluted with Triladyl, Andromed and Tris diluter, filled into 0.50 ml straws, sealed and equilibrated at 5℃ for freezing. The motility, viability and morphology were evaluated after dilution and in every step of cryopreservation (after equilibration, after freezing and after thawing). It became clear that motility and sperm viability of the frozen thawed semen differed significantly (p<0.05) but differed insignificantly (p>0.05) immediate after dilution among the diluters. The motility of frozen thawed semen was 42.50%±1.71, 34.67%±1.67 and 28.71%±1.55 in Triladyl, Andromed and Tris diluter, respectively. The viability of frozen thawed semen was 40.51%±1.78, 32.24%±1.52 and 26.84%±1.56 in Triladyl, Andromed and Tris diluter, respectively. In case of morphology, it differed insignificantly (p>0.05) among the diluters in every step of cryopreservation. Higher percentage of normal spermatozoa was found in Triladyl based diluter (87.11%) in frozen thawed semen. After 15 days of sample preparation, the motility of prepared sample was again checked to ensure the quality of frozen semen in liquid nitrogen (LN). It differed insignificantly (p>0.05) with frozen thawed semen after 24 hours of production. The obtained conception rate was 38% for Triladyl, 35% for Andromed and 27% for Tris diluter. The results of the study revealed that cryopreservation of buck semen revealed good results with Triladyl diluter followed by Andromed and Tris diluter. Bangladesh Journal of Animal Science 52 (4): 105-113

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稀释剂对孟加拉黑山羊冷冻精液产量的影响

本研究旨在调查三种稀释剂（Triladyl、Andromed 和 Tris）用于生产孟加拉黑山羊冷冻精液的功效及其对受孕率的影响。在 6 个月的时间里，收集了 6 头公羊的数据。采集的每份样本都用三乐定、安多美和三羟甲基氨基甲烷稀释剂稀释，装入 0.50 毫升的吸管中，密封并在 5℃下平衡后进行冷冻。稀释后和冷冻保存的每个步骤（平衡后、冷冻后和解冻后）都对精子的活力、存活率和形态进行了评估。结果表明，不同稀释剂稀释后，冷冻解冻精液的运动能力和精子存活率差异很大（P0.05）。冷冻解冻精液的运动能力在 Triladyl、Andromed 和 Tris 稀释剂中分别为 42.50%±1.71、34.67%±1.67 和 28.71%±1.55。冷冻解冻精液的存活率在 Triladyl、Andromed 和 Tris 稀释剂中分别为 40.51%±1.78、32.24%±1.52 和 26.84%±1.56。在冷冻保存的每个步骤中，不同稀释剂的精子形态差异均不显著（P>0.05）。在冷冻解冻的精液中，三苯甲基稀释液中正常精子的比例较高（87.11%）。样本制备 15 天后，再次检查制备样本的运动能力，以确保液氮（LN）冷冻精液的质量。结果表明，在生产 24 小时后，冷冻解冻精液的运动能力与冷冻解冻精液的运动能力相差不大（P>0.05）。Triladyl的受孕率为38%，Andromed为35%，Tris diluter为27%。研究结果表明，使用 Triladyl 稀释剂冷冻保存雄鹿精液效果良好，其次是 Andromed 和 Tris 稀释剂。孟加拉国动物科学杂志 52 (4)：105-113

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Bangladesh Journal of Animal Science

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