Quantification of X sperm by raman spectroscopy in percoll density gradient centrifuged buffalo semen

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2023-12-31 DOI:10.56825/bufbu.2023.4243847

Meenakshi Rawat, Mridula Sharma, Kamal Devlal, Dinesh Pandey, Harihar Prasad Gupta, M. Rana

引用次数: 0

Abstract

The present study was conducted to observe effect of percoll density gradient centrifugation of buffalo bull semen on quantity of X sperms. Ejaculates were collected by artificial vagina method. Semen with mass motility >+3 and progressive motility >70 % were selected for experiment. X sperm Enrichment of semen was done by discontinuous percoll density gradient centrifugation and three groups were formed ie Group 1 (3 layer 70%, 50% and 30%) Group 2 (7 layer 70%, 60%, 50%, 40%, 30%, 20% and 10%) Group 3 (7 layer 80%, 70%, 60%, 50%, 40%, 30% and 20%). Centrifugation of semen of three groups and control (fresh semen without gradients) was done. After centrifugation, the supernatant part was removed and the pellet of each group was used for X sperm enrichment assessment by Raman spectroscopy. Results revealed that X sperm enrichment was higher in the pellets of Group 2 followed Group 3, Group 1 and Control as Raman peaks on DNA specific bands corresponds to more number of x sperm were higher respectively.

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用拉曼光谱法确定水牛精液 percoll 密度梯度离心中 X 精子的数量

本研究旨在观察对水牛精液进行 percoll 密度梯度离心对 X 精子数量的影响。采用人工阴道法收集射精。实验选择了大量运动能力大于+3 和渐进运动能力大于 70% 的精液。精液中 X 精子的富集是通过不连续 percoll 密度梯度离心法进行的，共分为三组，即第一组（3 层 70%、50% 和 30%）、第二组（7 层 70%、60%、50%、40%、30%、20% 和 10%）、第三组（7 层 80%、70%、60%、50%、40%、30% 和 20%）。对三组精液和对照组（无梯度的新鲜精液）进行离心。离心后，去掉上清液部分，取各组的沉淀，用拉曼光谱评估 X 精子的富集情况。结果显示，第 2 组、第 3 组、第 1 组和对照组的颗粒中 X 精子富集度较高，因为 DNA 特定条带上的拉曼峰分别对应较多的 X 精子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.