Genetic Loss of miR-205 Causes Increased Mammary Gland Development

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2023-12-31 DOI:10.3390/ncrna10010004

A. Cataldo, Douglas G. Cheung, John Hagan, Matteo Fassan, Sukhinder Sandhu-Deol, Carlo M. Croce, Gianpiero di Leva, M. Iorio

引用次数: 0

Abstract

MiRNAs play crucial roles in a broad spectrum of biological processes, both physiological and pathological. Different reports implicate miR-205 in the control of breast stem cell properties. Differential miR-205 expression has been observed in different stages of mammary gland development and maturation. However, a functional role in this process has not been clearly demonstrated. We generated an miR-205 knockout in the FVB/N mouse strain, which is viable and characterized by enhanced mammary gland development. Indeed, mammary glands of miR-205 −/− female mice at different ages (1.5 and 5.5 months) show increased outgrowth and branching. This evidence is consistent with our previously reported data demonstrating the direct miR-205-mediated targeting of HER3, a master regulator of mammary gland development, and the oncosuppressive activity of this microRNA in different types of breast cancer.

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基因缺失 miR-205 会导致乳腺发育加快

MiRNA 在生理和病理等广泛的生物过程中发挥着至关重要的作用。不同的报告显示，miR-205 与乳腺干细胞特性的控制有关。在乳腺发育和成熟的不同阶段，已观察到不同的 miR-205 表达。然而，miR-205在这一过程中的功能性作用尚未得到明确证实。我们在 FVB/N 小鼠品系中产生了一种 miR-205 基因敲除小鼠，这种小鼠存活率高，乳腺发育增强。事实上，miR-205 -/-雌性小鼠在不同年龄段（1.5 个月和 5.5 个月）的乳腺显示出更多的外生和分支。这一证据与我们之前报道的数据一致，即 miR-205 介导的直接靶向 HER3（乳腺发育的主调控因子）以及该 microRNA 在不同类型乳腺癌中的抑制活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.