Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma

IF 3.1 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR

Inorganics Pub Date : 2024-01-01 DOI:10.3390/inorganics12010019

Ribhu Maity, Biplab Manna, Swapan Maity, Kalyanmoy Jana, T. Maity, Mohd Afzal, Nayim Sepay, B. Samanta

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引用次数: 0

Abstract

The current study provides an in-depth analysis of the biological properties of a Cu(II) complex (C22H24Cu2N6O10) obtained from an aryl-semicarbazone ligand derived (L) from the condensation of 2,4-dihydroxy acetophenone and semicarbazide. The binding behavior of this complex with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) protein was explored using a combination of experimental and theoretical approaches. The results suggest that the complex binds with CT-DNA via a partial intercalation, and hydrophobic interaction. However, the complex binds to BSA protein predominantly through hydrogen bonding or van der Waals interactions rather than hydrophobic interactions. The molecular docking methodology was carried out to substantiate the experimental finding. Furthermore, the in vitro cytotoxicity study was conducted on human cervix uteri carcinoma (SiHa cancerous cell) lines upon exposure to the complex, and the findings reveal a considerable decrease in cell viability, when compared to the control. Overall, this study provides a comprehensive understanding of the biological potential of the Cu(II) complex and its potential as an anti-cancer agent.

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基于芳基半胱肼的 Cu(II) 复合物与 DNA 和 BSA 的相互作用及对人子宫颈癌的抗癌活性的合成

本研究深入分析了一种 Cu(II) 复合物（C22H24Cu2N6O10）的生物特性，该复合物由 2,4- 二羟基苯乙酮和半咔嗪缩合而成的芳基半咔嗪配体 (L) 制得。研究人员采用实验和理论相结合的方法，探讨了这种复合物与小牛胸腺 DNA（CT-DNA）和牛血清白蛋白（BSA）蛋白的结合行为。结果表明，该复合物通过部分插层和疏水作用与 CT-DNA 结合。然而，复合物与 BSA 蛋白的结合主要是通过氢键或范德华相互作用，而不是疏水相互作用。分子对接方法证实了这一实验结果。此外，研究人员还对人类子宫颈癌（SiHa 癌细胞）细胞系接触复合物后进行了体外细胞毒性研究，结果显示，与对照组相比，细胞存活率大大降低。总之，这项研究让人们全面了解了 Cu（II）复合物的生物潜力及其作为抗癌剂的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inorganics Chemistry-Inorganic Chemistry

CiteScore

2.80

自引率

10.30%

发文量

193

审稿时长

6 weeks

期刊介绍： Inorganics is an open access journal that covers all aspects of inorganic chemistry research. Topics include but are not limited to: synthesis and characterization of inorganic compounds, complexes and materials structure and bonding in inorganic molecular and solid state compounds spectroscopic, magnetic, physical and chemical properties of inorganic compounds chemical reactivity, physical properties and applications of inorganic compounds and materials mechanisms of inorganic reactions organometallic compounds inorganic cluster chemistry heterogenous and homogeneous catalytic reactions promoted by inorganic compounds thermodynamics and kinetics of significant new and known inorganic compounds supramolecular systems and coordination polymers bio-inorganic chemistry and applications of inorganic compounds in biological systems and medicine environmental and sustainable energy applications of inorganic compounds and materials MD