OPTIMALISASI DAN UJI EFEKTIVITAS ACTINOMYCETES PADA Brassica chinensis DI BAWAH CEKAMAN KEKERINGAN DAN pH MASAM ULTISOL

Shendy Citra Oktaviana Dewi, Didik Suprayogo, D. Rahmanto, Tri Setia Rini
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Abstract

Drought stress and acid soil pH are a form of abiotic stress on acid-dry land. Actinomycetes are a group of transitional microbes that have benefits as bioprotectants, biostimulators, and biofertilizers. This study aimed to determine the optimum growth environment for Actinomycetes at various incubation temperatures (25, 30, 35, and 40 ℃) and media pH (5, 6, 7, 8, and 9) and prove its effectiveness on mustard plants when given drought stress, and increase in acid soil pH. The research was conducted in the LOB greenhouse of PT Great Giant Pineapple using two factors, namely Actinomycetes (F22, F26, F34, F39, and F47) and irrigation (watered and not watered). Observations included Actinomycetes population (CFU mL-1), plant response (percentage of stress, death, survival, recovery), plant growth (plant length, number of leaves, root length), plant production (fresh weight), soil pH, and soil organic matter. Results of this study showed that Actinomycetes F22 gave the highest total population (107 CFU mL-1), and the P4K1, P4K2, and P2K2 treatment combination gave the best results on the observed parameters.
干旱和酸碱度胁迫下氨甲蝶呤对白藜的优化和功效试验
干旱胁迫和酸性土壤 pH 值是酸性干旱土地上的一种非生物胁迫。放线菌是一类过渡性微生物,具有生物保护剂、生物刺激剂和生物肥料的功效。本研究旨在确定放线菌在不同培养温度(25、30、35 和 40 ℃)和培养基 pH 值(5、6、7、8 和 9)下的最佳生长环境,并证明其在干旱胁迫和酸性土壤 pH 值升高的情况下对芥菜植物的有效性。研究在 PT Great Giant Pineapple 的 LOB 温室中进行,使用了两个因素,即放线菌(F22、F26、F34、F39 和 F47)和灌溉(浇水和不浇水)。观察指标包括放线菌数量(CFU mL-1)、植物反应(胁迫百分比、死亡、存活、恢复)、植物生长(株长、叶片数、根长)、植物产量(鲜重)、土壤 pH 值和土壤有机质。研究结果表明,放线菌 F22 的总种群数(107 CFU mL-1)最高,P4K1、P4K2 和 P2K2 处理组合对观察到的参数效果最好。
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