The role of glucose and fructose on lipid droplet metabolism in human normal bronchial and cancer lung cells by Raman spectroscopy

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2023-12-29 DOI:10.1016/j.chemphyslip.2023.105375

Monika Kopec, Karolina Beton-Mysur

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引用次数: 0

Abstract

Fructose is one of the most important monosaccharides in the human diet that the human body needs for proper metabolism. This paper presents an approach to study biochemical changes caused by sugars in human normal bronchial cells (BEpiC) and human cancer lung cells (A549) by Raman spectroscopy and Raman imaging. Results after supplementation of human bronchial and lung cells with fructose are also discussed and compared with results obtained for pure human bronchial and lung cells. Based on Raman techniques we have proved that peaks at 750 cm⁻¹, 1126 cm⁻¹, 1444 cm⁻¹, 1584 cm⁻¹ and 2845 cm⁻¹ can be treated as biomarkers to monitor fructose changes in cells. Results for fructose have been compared with results for glucose. Raman analysis of the bands at 750 cm⁻¹, 1126 cm⁻¹, 1584 cm⁻¹ and 2845 cm⁻¹ for pure BEpiC and A549 cells and BEpiC and A549 after supplementation with fructose and glucose are higher after supplementation with fructose in comparison to glucose. The obtained results shed light on the uninvestigated influence of glucose and fructose on lipid droplet metabolism by Raman spectroscopy methods.

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通过拉曼光谱分析葡萄糖和果糖对人体正常支气管细胞和癌肺细胞脂滴代谢的作用

果糖是人体膳食中最重要的单糖之一，是人体正常代谢所需的物质。本文介绍了一种利用拉曼光谱和拉曼成像技术研究糖类在人类正常支气管细胞（BEpiC）和人类癌肺细胞（A549）中引起的生化变化的方法。我们还讨论了在人类支气管细胞和肺细胞中添加果糖后的结果，并将其与纯人类支气管细胞和肺细胞的结果进行了比较。基于拉曼技术，我们证明了 750 cm-1、1126 cm-1、1444 cm-1、1584 cm-1 和 2845 cm-1 处的峰值可作为生物标记来监测细胞中果糖的变化。果糖的检测结果与葡萄糖的检测结果进行了比较。对纯 BEpiC 和 A549 细胞以及添加果糖和葡萄糖后的 BEpiC 和 A549 细胞在 750 cm-1、1126 cm-1、1584 cm-1 和 2845 cm-1 处的条带进行拉曼分析，发现添加果糖后的条带比添加葡萄糖后的条带高。这些结果揭示了拉曼光谱方法尚未研究的葡萄糖和果糖对脂滴代谢的影响。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.