Equine chorionic gonadotropin treatment and timed artificial insemination for dairy cow production under heat stress

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-30 DOI:10.1262/jrd.2023-069

Daisuke FUNAKOSHI, Hidetoshi SHIOTANI, Makoto SEKI

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Abstract

This study investigated the effects of timed artificial insemination (TAI) and equine chorionic gonadotropin (eCG) administration on lactating dairy cows under heat-stress conditions (average temperature-humidity index: 80). Timed artificial insemination was performed on the cows with (n = 57) or without (control, n = 41) supplementation with 500 IU of eCG at the day of PGF_2α treatment using the CIDR-Ovsynch protocol. GnRH was administered, and a progesterone device (CIDR) was inserted on Day −10 of the treatment protocol. The CIDR was removed on Day −3, and the cows were treated with PGF_2α. Two days later, a 2^nd GnRH injection was administered. Subsequently, AI was performed on Day 0 (16–20 h after the 2^nd GnRH injection), and pregnancy was diagnosed on Days 32 and 60. Plasma progesterone (P₄) concentrations were measured after AI. Results showed that the eCG group had a higher pregnancy per AI (P/AI) than the control group (43.9 vs. 12.2%, P = 0.002), which was also accompanied by elevated P₄ levels. Four cows in the eCG group had multiple calves, representing 7.0 and 16.0% of the group and pregnant cows, respectively. In conclusion, 500 IU of eCG combined with CIDR-Ovsynch in lactating dairy cows under severe heat stress conditions successfully improved fertility. However, the protocol may have a slight risk of multiple births.

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马绒毛膜促性腺激素治疗和定时人工授精用于热应激下的奶牛生产

本研究探讨了在热应激条件下（平均温湿度指数：80），定时人工授精（TAI）和注射马绒毛膜促性腺激素（eCG）对泌乳奶牛的影响。采用 CIDR-Ovsynch 方案对奶牛进行定时人工授精，在 PGF2α 处理当天补充（n = 57）或不补充（对照组，n = 41）500 IU eCG。注射 GnRH，并在治疗方案的第 -10 天植入黄体酮装置（CIDR）。第-3天取出CIDR，然后用PGF2α对奶牛进行治疗。两天后，进行第二次 GnRH 注射。随后，在第 0 天（第 2 次注射 GnRH 后 16-20 小时）进行人工授精，并在第 32 天和第 60 天确诊妊娠。人工授精后测量血浆孕酮（P4）浓度。结果显示，eCG组的每次人工授精妊娠率（P/AI）高于对照组（43.9% vs. 12.2%，P = 0.002），P4水平也随之升高。eCG 组中有四头奶牛产下多胎，分别占该组奶牛和怀孕奶牛的 7.0% 和 16.0%。总之，在严重热应激条件下，500 IU eCG 与 CIDR-Ovsynch 结合使用可成功提高泌乳奶牛的繁殖力。不过，该方案可能会有轻微的多胎风险。图表摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.