THE INFLUENCE OF THE WAR FACTOR ON AGE CHANGES IN THE SUBPOPULATION STRUCTURE OF LEUCOCYTES AS A COMPONENT OF INFLAMMAGING IN THE GROUPS OF MILITARY PERSONNEL AND CLEAN-UP WORKERS OF THE CHORNOBYL ACCIDENT.

L M Zvarych, V V Panchenko, N A Golyarnik, O A Belayev, D A Bazyka
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引用次数: 0

Abstract

Objective: to evaluate the subpopulation structure of peripheral blood leukocytes as a component of inflammaging in the servicemen of the Armed Forces of Ukraine and clean-up workers of the accident at the Chornobyl nuclear power plant.

Materials and methods: Three hundred ninety-seven males aged 30-82 (54.99 ± 8.85) years examined. Among them: clean-up workers of the accident at the Chornobyl nuclear power plant, aged 54-64 (59.95 ± 1.88) years, who were examined before the beginning and during the Russian military invasion of Ukraine; 24 civilians of the control group, aged 50-77 (58.87 ± 7.18) years and 15 persons of the age control group, aged 42-49 (46.00 ± 2.26) years, who examined before the war has started; the comparison group-W - 27 civilians (age: 51-82 (65.44 ± 9.13)) and the age control group-W - 14 civilians (age: 31-49 (40.64 ± 5.59)) who examined after the war has started; 73 servicemen of the Armed Forces of Ukraine aged 30-49 (42.20 ± 5.35) years and 54 servicemen aged 50-59 (54.07 ± 2.86) years. The subpopulation distribution of peripheral blood leukocytes was analyzed using flow cytometry.

Results: A decrease in the relative number of CD45+ 14- granulocytes, CD3+ HLA-DR+ T-lymphocytes, and CD3-57+ NK against the background of an increase in the number of CD45+ 14+ monocytes, CD3-HLA-DR+ B-lymphocytes found in the majority of people in the examination groups, the study of which conducted after the beginning of the war, CD4+ T-lymphocytes and CD3+ 57+ NKT. The relative number of CD45+ 14- granulocytes increased against the background of a decrease in CD45+ 14- lymphocytes was noted in clean-up workers who were examined before the war started.About 30% of civilians over 50 years of age who were examined before the war started had a decrease in CD19+ B- lymphocytes, CD4+ and CD8+ T-lymphocytes, CD3-57+ NK, CD3+ 57+ NKT, and the CD4+ / CD8+ ratio. Conducted correlation analysis revealed a weak to moderate inverse correlation between the relative number of CD19+ B-lymphocytes and age in civilians, regardless of when they were examined, and the servicemen of the Armed Forces of Ukraine.Inverse correlations of HLA-DR+ B-lymphocytes, CD45+ 14+ monocytes, CD4+ T-lymphocytes, CD4+ / CD8+ ratio, and CD3-57+ NK and CD3+ 57+ NKT with age were noted in civilians who were examined after the war has started. A direct correlation between CD3+ 57+ NKT and age was found in the servicemen of the Armed Forces of Ukraine.

Conclusions: The immunological profile of both clean-up workers in the Chornobyl nuclear power plant and the servicemen of the Armed Forces of Ukraine or civilians was similar compared to the group with reference indicators. Anintergroup study revealed changes in the subpopulation distribution of peripheral blood leukocytes, which may be associated with both aging and changes in living conditions during military operations. Specific changes in cellular immunity can either tip the balance towards inflammaging or indicate already existing immunopathologicalreactions.

战争因素对作为切尔诺贝利核电站事故军事人员和清理工人群体炎症组成部分的白细胞亚群结构的年龄变化的影响。
目的:评估乌克兰武装部队军人和切尔诺贝利核电站事故清理人员外周血白细胞亚群结构,作为炎症反应的组成部分:研究对象为 397 名男性,年龄在 30-82 岁之间(54.99 ± 8.85)。其中:切尔诺贝利核电站事故清理工人,54-64 岁(59.95 ± 1.88),在俄罗斯军事入侵乌克兰开始之前和期间接受检查;对照组 24 名平民,50-77 岁(58.87 ± 7.18);年龄对照组 15 人,42-49 岁(46.00 ± 2.在战争开始前接受检查的对比组-W-27 名平民(年龄:51-82(65.44±9.13)岁)和年龄对照组-W-14 名平民(年龄:31-49(40.64±5.59)岁);在战争开始后接受检查的乌克兰武装部队 73 名军人(年龄:30-49(42.20±5.35)岁)和 54 名军人(年龄:50-59(54.07±2.86)岁)。使用流式细胞术分析了外周血白细胞的亚群分布:结果:在战争开始后进行的研究中发现,在大多数检查组中,CD45+ 14+ 单核细胞、CD3-HLA-DR+ B 淋巴细胞、CD4+ T 淋巴细胞和 CD3+ 57+ NKT 的数量增加的背景下,CD45+ 14- 粒细胞、CD3+ HLA-DR+ T 淋巴细胞和 CD3-57+ NK 的相对数量减少。在战争开始前接受检查的清洁工人中,CD45+ 14- 粒细胞的相对数量有所增加,而 CD45+ 14- 淋巴细胞的数量则有所减少。在战争开始前接受检查的 50 岁以上的平民中,约有 30% 的人的 CD19+ B- 淋巴细胞、CD4+ 和 CD8+ T 淋巴细胞、CD3-57+ NK、CD3+ 57+ NKT 以及 CD4+ / CD8+ 比率均有所下降。相关性分析表明,无论何时接受检查,平民和乌克兰武装部队军人的 CD19+ B 淋巴细胞相对数量与年龄之间都存在弱到中等程度的反相关性。在战争开始后接受检查的平民中,HLA-DR+ B 淋巴细胞、CD45+ 14+ 单核细胞、CD4+ T 淋巴细胞、CD4+/CD8+ 比率、CD3-57+ NK 和 CD3+ 57+ NKT 与年龄呈反向相关。乌克兰武装部队军人的 CD3+ 57+ NKT 与年龄直接相关:切尔诺贝利核电站清理工人、乌克兰武装部队军人或平民的免疫学特征与参考指标组相似。一项组间研究显示,外周血白细胞亚群分布发生了变化,这可能与老化和军事行动期间生活条件的变化有关。细胞免疫的特定变化既可能使天平向炎症方向倾斜,也可能表明已经存在免疫病理反应。
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Problemy radiatsiinoi medytsyny ta radiobiolohii
Problemy radiatsiinoi medytsyny ta radiobiolohii Medicine-Radiology, Nuclear Medicine and Imaging
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