Sara Khosravinia, Saeid Malekzadeh-Shafaroudi, Abdolreza Bagheri, Assieh Behdad, Nasrin Moshtaghi
{"title":"Bioprospecting of Ten Microalgae Species Isolated from Saline Water Lake for Evaluation of the Biodiesel Production","authors":"Sara Khosravinia, Saeid Malekzadeh-Shafaroudi, Abdolreza Bagheri, Assieh Behdad, Nasrin Moshtaghi","doi":"10.1007/s12155-023-10707-2","DOIUrl":null,"url":null,"abstract":"<div><p>Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saline water lake. <i>Chlorella vulgaris</i>, <i>Chlorella sorokiniana</i>, <i>Chlamydomonas raudensis</i>, <i>Chlamydomonas hedleyi</i>, <i>Dunaliella salina</i>, <i>Picochlorum bazangan</i> sp. nov<i>.</i>, <i>Tetraselmis bazangan</i> sp. nov., <i>Haematococcus lacustris</i>, <i>Nannochloropsis oceanic</i>, and <i>Scenedesmus rubescens</i> were isolated and identified using 18SrDNA and <i>tuf</i>A markers. Biodiesel potentials were assayed by the determination of biomass productivity, biochemical components, fatty acid profile, and biodiesel properties. The results showed that the maximum biomass yield (1.22 g/L) belonged to <i>C. vulgaris</i>. The highest protein, carbohydrate, chlorophyll, and carotenoid content were recorded in <i>C. vulgaris</i>, <i>C. raudensis</i>, <i>C. sorokiniana</i>, and <i>D. salina</i>, respectively. <i>N. oceanica</i> accumulated high lipid content and omega-3 fractions (31.09%). However, <i>C. hedleyi</i> had the highest lipid productivity (11.64 g/L/day) compared to other microalgae. The best species for biodiesel production was <i>C. vulgaris</i>, with a specific growth rate of 0.36 day<sup>−1</sup>, lipid productivity of 7.45 g/L/day, and C16-C18 fatty acid profile of 78.3%. The microalgae <i>C. vulgaris</i> had appropriate biodiesel properties of low viscosity (4.49), high cetane number (55.38), and relatively low cloud point (4.98). Another choice was <i>N. oceanic</i>, with high lipid productivity, cetane number (59.79), oxidative stability (56.43), and low iodine value (47.11). Microalgae <i>T. bazangan</i> sp. nov. had a cetane number (55.24), low cloud point (4.71), and C16-C18 fatty acid profile of 82.34%. Accordingly, <i>C. vulgaris</i>, <i>T. bazangan</i> sp. nov., and <i>N. oceanic</i> can be considered potential species for biodiesel.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioEnergy Research","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s12155-023-10707-2","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENERGY & FUELS","Score":null,"Total":0}
引用次数: 0
Abstract
Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saline water lake. Chlorella vulgaris, Chlorella sorokiniana, Chlamydomonas raudensis, Chlamydomonas hedleyi, Dunaliella salina, Picochlorum bazangan sp. nov., Tetraselmis bazangan sp. nov., Haematococcus lacustris, Nannochloropsis oceanic, and Scenedesmus rubescens were isolated and identified using 18SrDNA and tufA markers. Biodiesel potentials were assayed by the determination of biomass productivity, biochemical components, fatty acid profile, and biodiesel properties. The results showed that the maximum biomass yield (1.22 g/L) belonged to C. vulgaris. The highest protein, carbohydrate, chlorophyll, and carotenoid content were recorded in C. vulgaris, C. raudensis, C. sorokiniana, and D. salina, respectively. N. oceanica accumulated high lipid content and omega-3 fractions (31.09%). However, C. hedleyi had the highest lipid productivity (11.64 g/L/day) compared to other microalgae. The best species for biodiesel production was C. vulgaris, with a specific growth rate of 0.36 day−1, lipid productivity of 7.45 g/L/day, and C16-C18 fatty acid profile of 78.3%. The microalgae C. vulgaris had appropriate biodiesel properties of low viscosity (4.49), high cetane number (55.38), and relatively low cloud point (4.98). Another choice was N. oceanic, with high lipid productivity, cetane number (59.79), oxidative stability (56.43), and low iodine value (47.11). Microalgae T. bazangan sp. nov. had a cetane number (55.24), low cloud point (4.71), and C16-C18 fatty acid profile of 82.34%. Accordingly, C. vulgaris, T. bazangan sp. nov., and N. oceanic can be considered potential species for biodiesel.
期刊介绍:
BioEnergy Research fills a void in the rapidly growing area of feedstock biology research related to biomass, biofuels, and bioenergy. The journal publishes a wide range of articles, including peer-reviewed scientific research, reviews, perspectives and commentary, industry news, and government policy updates. Its coverage brings together a uniquely broad combination of disciplines with a common focus on feedstock biology and science, related to biomass, biofeedstock, and bioenergy production.