Long and Short-Term Effect of mTOR Regulation on Cerebral Organoid Growth and Differentiations.

IF 4.4 4区医学 Q2 CELL & TISSUE ENGINEERING

Tissue engineering and regenerative medicine Pub Date : 2024-01-01 Epub Date: 2023-12-28 DOI:10.1007/s13770-023-00611-3

Sung Bum Park, Byungho Lim, Ki Young Kim, Byumseok Koh

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引用次数: 0

Abstract

Background: The mammalian target of rapamycin (mTOR) signaling is critical for the maintenance and differentiation of neurogenesis, and conceivably for many other brain developmental processes. However, in vivo studies of mTOR functions in the brain are often hampered due to the essential role of the associated signaling in brain development.

Methods: We monitored the long- and short-term effects of mTOR signaling regulation on cerebral organoids growth, differentiation and function using an mTOR inhibitor (everolimus) and an mTOR activator (MHY1485).

Results: Short-term treatment with MHY1485 induced faster organoid growth and differentiation, while long-term treatment induced the maturation of cerebral organoids.

Conclusion: These data suggest that the optimal activity of mTOR is crucial in maintaining normal brain development, and its role is not confined to the early neurogenic phase of brain development.

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mTOR调控对脑器质性组织生长和分化的长期和短期影响

背景：哺乳动物雷帕霉素靶标（mTOR）信号传导对神经发生的维持和分化至关重要，而且可以想象对许多其他脑发育过程也至关重要。然而，由于相关信号在大脑发育中的重要作用，对大脑中 mTOR 功能的体内研究往往受到阻碍：方法：我们使用mTOR抑制剂（依维莫司）和mTOR激活剂（MHY1485）监测了mTOR信号调节对脑器官组织生长、分化和功能的长期和短期影响：结果：MHY1485的短期治疗诱导了更快的类器官生长和分化，而长期治疗则诱导了脑类器官的成熟：这些数据表明，mTOR的最佳活性对维持大脑的正常发育至关重要，而且它的作用并不局限于大脑发育的早期神经源阶段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue engineering and regenerative medicine CELL & TISSUE ENGINEERING-ENGINEERING, BIOMEDICAL

CiteScore

6.80

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Tissue Engineering and Regenerative Medicine (Tissue Eng Regen Med, TERM), the official journal of the Korean Tissue Engineering and Regenerative Medicine Society, is a publication dedicated to providing research- based solutions to issues related to human diseases. This journal publishes articles that report substantial information and original findings on tissue engineering, medical biomaterials, cells therapy, stem cell biology and regenerative medicine.