The effect of hyperglycemia on the activation of peritoneal macrophages of albino rats.

Q3 Biochemistry, Genetics and Molecular Biology
I A Golyako, V S Kuzmin, L R Gorbacheva
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引用次数: 0

Abstract

Hyperglycemia is one of the main damaging factors of diabetes mellitus (DM). The severity of this disease is most clearly manifested under conditions of the inflammatory process. In this work, we have studied the activation features of rat peritoneal macrophages (MPs) under conditions of high glucose concentration in vitro. Comparison of the independent and combined effects of streptozotocin-induced DM and hyperglycemia on proliferation and accumulation of nitrites in the MPs culture medium revealed similarity of their effects. Elevated glucose levels and, to a lesser extent, DM decreased basal proliferation and NO production by MPs in vitro. The use of the protein kinase C (PKC) activator, phorbol ester (PMA), abolished the proinflammatory effect of thrombin on PMs. This suggests the involvement of PKC in the effects of the protease. At the same time, the effect of thrombin on the level of nitrites in the culture medium demonstrates a pronounced dose-dependence, which was not recognized during evaluation of proliferation. Proinflammatory activation of MPs is potentiated by hyperglycemia, one of the main pathological factors of diabetes. Despite the fact that high concentrations of glucose have a significant effect on proliferation and NO production, no statistically significant differences were found between the responses of MPs obtained from healthy animals and from animals with streptozotocin-induced DM. This ratio was observed for all parameters studied in the work, during analysis of cell proliferation and measurement of nitrites in the culture medium. Thus, the results obtained indicate the leading role of elevated glucose levels in the regulation of MPs activation, which is comparable to the effect of DM and even "masks" it.

高血糖对白化大鼠腹腔巨噬细胞活化的影响
高血糖是糖尿病(DM)的主要损害因素之一。这种疾病的严重性在炎症过程中表现得最为明显。在这项工作中,我们研究了大鼠腹腔巨噬细胞(MPs)在体外高浓度葡萄糖条件下的活化特征。比较链脲佐菌素诱导的 DM 和高血糖对 MPs 增殖和亚硝酸盐在 MPs 培养基中积累的独立影响和联合影响,发现它们的影响具有相似性。葡萄糖水平升高和 DM(在较小程度上)降低了体外中枢神经系统的基础增殖和 NO 生成。使用蛋白激酶 C(PKC)激活剂--光稳定剂(PMA)可消除凝血酶对 PMs 的促炎作用。这表明PKC参与了蛋白酶的作用。同时,凝血酶对培养基中亚硝酸盐水平的影响表现出明显的剂量依赖性,这在增殖评估过程中没有被发现。糖尿病的主要病理因素之一是高血糖,而高血糖会增强中性粒细胞的促炎激活作用。尽管高浓度葡萄糖对增殖和氮氧化物的产生有显著影响,但从健康动物和链脲佐菌素诱导的糖尿病动物身上获得的 MPs 的反应在统计学上没有发现显著差异。在分析细胞增殖和测量培养基中亚硝酸盐的过程中,在工作中研究的所有参数中都观察到了这一比率。因此,所获得的结果表明,葡萄糖水平升高在中枢神经系统活化的调节中起着主导作用,其作用与 DM 的作用相当,甚至 "掩盖 "了 DM 的作用。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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