Human epithelial lung cell toxicity assessment of collected graphite particles from an iron casting industry (in vitro study).

IF 2.2 4区医学 Q3 ENVIRONMENTAL SCIENCES

International Journal of Environmental Health Research Pub Date : 2024-09-01 Epub Date: 2023-12-26 DOI:10.1080/09603123.2023.2298248

Rezvan Zendehdel, Omar Hahad, Zahra Panjali

{"title":"Human epithelial lung cell toxicity assessment of collected graphite particles from an iron casting industry (in vitro study).","authors":"Rezvan Zendehdel, Omar Hahad, Zahra Panjali","doi":"10.1080/09603123.2023.2298248","DOIUrl":null,"url":null,"abstract":"Workers in the iron casting industries are exposed to various chemicals, especially graphite in furnace process. This study aims to investigate the toxic effects of graphite particles on human lung cells. Particle characteristics were confirmed by electron microscope and light scattering. Cell viability and oxidative stress markers were measured. The expression of oxidative repair genes, namely OGG1, MTH1, and ITPA, was evaluated. The average particle size was determined to be 172.1 ± 11.96 nm. The median inhibition concentration (IC50) of graphite particles was 46.75 µg/mL. Notably, 25 and 50 µg/mL concentrations resulted in significant GSH depletion and MDA production. The high concentration of graphite particles (200 µg/mL) led to OGG1 suppression and increased MTH1 expression. Based on these findings, graphite exposure may induce toxicity in human lung cells by increasing oxidative stress. Further research is necessary to fully understand the mechanisms underlying graphite toxicity.","PeriodicalId":14039,"journal":{"name":"International Journal of Environmental Health Research","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Environmental Health Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09603123.2023.2298248","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/26 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Workers in the iron casting industries are exposed to various chemicals, especially graphite in furnace process. This study aims to investigate the toxic effects of graphite particles on human lung cells. Particle characteristics were confirmed by electron microscope and light scattering. Cell viability and oxidative stress markers were measured. The expression of oxidative repair genes, namely OGG1, MTH1, and ITPA, was evaluated. The average particle size was determined to be 172.1 ± 11.96 nm. The median inhibition concentration (IC₅₀) of graphite particles was 46.75 µg/mL. Notably, 25 and 50 µg/mL concentrations resulted in significant GSH depletion and MDA production. The high concentration of graphite particles (200 µg/mL) led to OGG1 suppression and increased MTH1 expression. Based on these findings, graphite exposure may induce toxicity in human lung cells by increasing oxidative stress. Further research is necessary to fully understand the mechanisms underlying graphite toxicity.

查看原文本刊更多论文

从铸铁业收集的石墨颗粒对人类上皮肺细胞的毒性评估（体外研究）。

铸铁行业的工人在炼炉过程中会接触到各种化学物质，尤其是石墨。本研究旨在探讨石墨颗粒对人体肺部细胞的毒性影响。通过电子显微镜和光散射法确认了石墨颗粒的特征。测量了细胞活力和氧化应激标记物。评估了氧化修复基因（即 OGG1、MTH1 和 ITPA）的表达。测定的平均粒径为 172.1 ± 11.96 nm。石墨颗粒的中位抑制浓度（IC50）为 46.75 µg/mL。值得注意的是，25 微克/毫升和 50 微克/毫升的浓度会导致严重的 GSH 消耗和 MDA 生成。高浓度石墨颗粒（200 微克/毫升）导致 OGG1 抑制和 MTH1 表达增加。基于这些发现，石墨暴露可能会通过增加氧化应激而诱发人类肺细胞的毒性。要全面了解石墨毒性的内在机制，还需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Environmental Health Research 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.70

自引率

3.10%

发文量

134

审稿时长

>12 weeks

期刊介绍： International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.

文献相关原料

公司名称	产品信息	采购帮参考价格