PLUM POX VIRUS STRAINS IDENTIFIED IN PEACH IN SOUTH CENTRAL REGION OF BULGARIA

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2023-12-12 DOI:10.33045/fgr.v39.2023.18

S. Milusheva, Nataliya Dimitrova, Svetla Pandova

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引用次数: 0

Abstract

Sharka disease, caused by plum pox virus (PPV), was detected on peach (Prunus persica (L.) Batsch.) for the first time in Bulgaria in 1989. Nowadays PPV is widespread in peach crops grown under varied agro-ecological conditions. The current study aimed to determinate the PPV strains spread in peach in six agro-ecological micro-regions located in three provinces (Plovdiv, Pazardzhik, Stara Zagora) of South Bulgaria. Altogether 70 PPV accessions, collected from 23 peach cultivars, were analysed by reverse transcription – polymerase chain reaction (RT-PCR) using primer pairs discriminating isolates of PPV-M, PPV-D and PPV-Rec strains. The summarized results revealed that PPV-M and PPV-D were identified in 85.7% and 8.6 % of the analysed samples, respectively. Both strains were detected at the highest infection rates in Pazardzhik province. Co-infection by both strains PPV-M and PPV-D was registered at the rate of 5.7 % of the tested accessions. PPV-Rec strain was not identified neither single nor in co-infection with the other strains. It has found differences in strain status in each of the studied orchards located in one and the same province. Likely the continuous increase of the PPV infection rate in peach is due to the prevalence of PPV-M strain, considered the epidemic form of the virus, and possible arising of higher virulent PPV-D variants. In that case, it should be applied all reasonable measures for containment of the virus spread and protection of the tree health.

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保加利亚中南部地区桃子中发现的李痘病毒株系

1989 年，保加利亚首次在桃（Prunus persica (L.) Batsch.）上发现了由李痘病毒（PPV）引起的 Sharka 病。如今，PPV 在不同农业生态条件下种植的桃树上广泛传播。本研究旨在确定 PPV 菌株在南保加利亚三个省（普罗夫迪夫、帕扎尔日克、斯塔拉扎戈拉）六个农业生态微区的桃中的传播情况。通过反转录聚合酶链反应（RT-PCR）分析了从 23 个桃栽培品种中收集到的 70 个 PPV 抗原，并使用引物对 PPV-M、PPV-D 和 PPV-Rec 株系的分离物进行了鉴别。汇总结果显示，85.7% 和 8.6% 的分析样本中分别鉴定出 PPV-M 和 PPV-D。这两种菌株在帕扎尔日克省的感染率最高。同时感染 PPV-M 和 PPV-D 株系的比例为 5.7%。没有发现 PPV-Rec 株系单独感染或与其他株系共同感染。在同一个省的每个研究果园中，都发现了不同的菌株状态。桃子中 PPV 感染率的持续上升可能是由于 PPV-M 株（被认为是该病毒的流行形式）的流行，以及可能出现了毒性更强的 PPV-D 变种。在这种情况下，应采取一切合理措施遏制病毒传播，保护树木健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.