Safety assessment of Rhodobacter azotoformans SY5 for potential application in Chinese mitten crab Eriocheir sinensis

IF 3 4区医学 Q2 MICROBIOLOGY

Beneficial microbes Pub Date : 2023-12-13 DOI:10.1163/18762891-20230086

H. Cao, S. Zhang, X. Zheng, L. Xu, J. Diao, Y. Wang, C. Gai, H. Ye

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引用次数: 0

Abstract

Rhodobacter species are promising beneficial microbes that can improve growth performance, immunity and antioxidant capability in aquatic crustaceans. Yet the safety of Rhodobacter azotoformans for potential application in Chinese mitten crab Eriocheir sinensis is still unclear. In the present study, R. azotoformans SY5, a potential probiotic additive that can significantly improve the growth performance, immunity, antioxidant capability, and disease resistance in E. sinensis, was evaluated for safety through whole genome sequencing, antibiotic resistance, toxic metabolites, virulence, and crab tolerance assays. The results indicated that R. azotoformans SY5 only harboured the acyl carrier protein-encoding gene (acpP) that was universal in probiotic bacteria with the function of bacterial fatty acid biosynthesis, exhibited high susceptibility to aminoglycosides, penicillins, polymyxins, polyphosphates, quinolones, and tetracyclines antimicrobials, and possessed inability to produce hemolysin, hydrogen sulphide, nitrite, ammonia, and phenylpyruvate. In addition, R. azotoformans SY5 showed no pathogenicity for E. sinensis with the seven-day acute intraperitoneal LD50 value of above 6.0 × 109 cfu/ml and 30-day chronic oral LD50 of above 6.0 × 109 cfu/g diet. To our knowledge, this is the first report on the safety of R. azotoformans for potential application in Chinese mitten crabs.

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对可能应用于中华绒螯蟹的氮化罗氏菌 SY5 的安全性评估

罗氏菌是一种很有前途的有益微生物，可以提高水生甲壳类动物的生长性能、免疫力和抗氧化能力。然而，在中华绒螯蟹中潜在应用的氮基多变形罗氏菌的安全性仍不清楚。在本研究中，通过全基因组测序、抗生素耐药性、有毒代谢物、毒力和蟹耐受性实验，对可显著改善中华绒螯蟹生长性能、免疫力、抗氧化能力和抗病能力的潜在益生菌添加剂--R. azotoformans SY5进行了安全性评估。结果表明，R.对氨基糖苷类、青霉素类、多粘菌素类、多磷酸盐类、喹诺酮类和四环素类抗菌药物具有高度敏感性，不能产生溶血素、硫化氢、亚硝酸盐、氨和苯丙酮酸。此外，R. azotoformans SY5 对中华大肠杆菌无致病性，其七天急性腹腔 LD50 值高于 6.0 × 109 cfu/ml，30 天慢性口服 LD50 值高于 6.0 × 109 cfu/g 食物。据我们所知，这是第一份关于R. azotoformans在中华绒螯蟹中潜在应用安全性的报告。

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来源期刊

Beneficial microbes MICROBIOLOGY-NUTRITION & DIETETICS

CiteScore

7.90

自引率

1.90%

发文量

审稿时长

>12 weeks

期刊介绍： Beneficial Microbes is a peer-reviewed scientific journal with a specific area of focus: the promotion of the science of microbes beneficial to the health and wellbeing of man and animal. The journal contains original research papers and critical reviews in all areas dealing with beneficial microbes in both the small and large intestine, together with opinions, a calendar of forthcoming beneficial microbes-related events and book reviews. The journal takes a multidisciplinary approach and focuses on a broad spectrum of issues, including safety aspects of pro- & prebiotics, regulatory aspects, mechanisms of action, health benefits for the host, optimal production processes, screening methods, (meta)genomics, proteomics and metabolomics, host and bacterial physiology, application, and role in health and disease in man and animal. Beneficial Microbes is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as those of policy makers and regulators. The journal will have five major sections: * Food, nutrition and health * Animal nutrition * Processing and application * Regulatory & safety aspects * Medical & health applications In these sections, topics dealt with by Beneficial Microbes include: * Worldwide safety and regulatory issues * Human and animal nutrition and health effects * Latest discoveries in mechanistic studies and screening methods to unravel mode of action * Host physiology related to allergy, inflammation, obesity, etc. * Trends in application of (meta)genomics, proteomics and metabolomics * New developments in how processing optimizes pro- & prebiotics for application * Bacterial physiology related to health benefits