Fungal Endophytes of Moringa (Moringa oleifera L.), Neem (Azadirachta indica) and Lavender (Lavandula angustifolia) and Their Biological Control of Fusarium Wilt of Banana

IF 2.1 Q3 MICROBIOLOGY

Microbiology Research Pub Date : 2023-12-15 DOI:10.3390/microbiolres14040143

Bonface M. Nthuku, Esther W. Kahariri, Johnson K. Kinyua, Evans N. Nyaboga

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引用次数: 0

Abstract

Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), significantly affects the productivity of the banana crop in the field. Currently, there are no effective control measures available, and therefore, there is an urgent need to develop novel strategies to control the spread of the disease. Biological control is a promising strategy for the management and control of the disease. The aim of this study was to identify fungal endophytes associated with Moringa (Moringa oleifera), Neem tree (Azadirachta indica) and Lavender (Lavandula angustifolia) and their antifungal activities against Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). We isolated 69 fungal endophytes from different tissues of M. oleifera, A. indica and L. angustifolia and screened for antifungal activity against Foc TR4. Six fungal endophytes exhibited highest antagonistic activity against Foc TR4 based on dual-culture assays. Based on morphology and ITS gene sequence analysis, the selected six endophytes were identified to be related to Alternaria alternata (MB7 and NR3), Neofusicoccum parvum (LB1), Fusarium oxysporum (LR1), Talaromyces amestolkiae (MB14) and Alternaria tenuissima (NB6). The culture filtrates of the six fungal endophytes (LB1, LR1, MB7, MB14, NB6 and NR3) exhibited more than 50% inhibition of mycelial growth of Foc TR4 in vitro and were producers of β-1,3-glucanase. The six fungal endophytes showed biocontrol efficacy against Fusarium wilt in pot experiments. The findings from this study demonstrate that fungal endophytes LB1, LR1, MB7, MB14, NB6 and NR3 should be explored as biocontrol agents and biofertilizers in banana production.

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辣木（Moringa oleifera L.）、印楝（Azadirachta indica）和薰衣草（Lavandula angustifolia）的真菌内生菌及其对香蕉镰刀菌枯萎病的生物防治作用

由 Fusarium oxysporum f. sp. cubense（Foc）引起的镰刀菌枯萎病严重影响了香蕉作物的产量。目前还没有有效的控制措施，因此迫切需要开发新的战略来控制该疾病的传播。生物防治是管理和控制该疾病的一种有前途的策略。本研究的目的是鉴定与辣木（Moringa oleifera）、印度楝树（Azadirachta indica）和薰衣草（Lavandula angustifolia）相关的真菌内生菌，以及这些真菌内生菌对 Fusarium oxysporum f. sp. cubense tropical race 4（Foc TR4）的抗真菌活性。我们从 M.oleifera、A. indica 和 L. angustifolia 的不同组织中分离出 69 种真菌内生菌，并筛选出它们对 Foc TR4 的抗真菌活性。根据双重培养试验，6 种真菌内生菌对 Foc TR4 的拮抗活性最高。根据形态学和 ITS 基因序列分析，所选的六种内生真菌被鉴定为与交替丝核菌（MB7 和 NR3）、Neofusicoccum parvum（LB1）、Fusarium oxysporum（LR1）、Talaromyces amestolkiae（MB14）和 Alternaria tenuissima（NB6）有关。六种真菌内生菌（LB1、LR1、MB7、MB14、NB6 和 NR3）的培养滤液在体外对 Foc TR4 菌丝生长的抑制率超过 50%，并能产生 β-1,3-葡聚糖酶。在盆栽实验中，这六种真菌内生菌对镰刀菌枯萎病具有生物防治效果。这项研究结果表明，真菌内生菌 LB1、LR1、MB7、MB14、NB6 和 NR3 应作为生物防治剂和生物肥料在香蕉生产中进行开发。

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.