Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2023-12-14 DOI:10.1134/S0003683823080045

A. V. Boyko, E. A. Mikheeva, T. L. Zakharova, N. A. Osina

引用次数: 0

Abstract

An instrument-free method for detecting the ability of Vibrio cholerae strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.

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用点吸法检测培养液中的霍乱毒素

我们开发了一种无需仪器的方法来检测霍乱弧菌菌株产生毒素的能力。该方法基于在浸渍棒上进行的点式酶联免疫吸附。浸渍棒所需的单克隆抗体比微孔板 ELISA 少 100 倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.