Variovorax soli mediated growth amelioration and abiotic stress alleviation in Capsicum annuum

IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology
A. Maxton, S. Masih
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引用次数: 0

Abstract

In present study, rhizobacterial strains were isolated from soil sample and screened for plant growth promoting and antagonistic potential in vitro condition. The isolate exhibiting maximum plant growth promoting and antagonistic potential was subjected to biochemical characterization and 16S rDNA gene sequencing. Based on molecular characterization, the isolate was identified as Variovorax soli with NCBI accession number LC372534. The PGP potential of Variovorax soli was established as it augmented plant growth in control and under salt, drought, heavy metal stress conditions. Positive results for in-vitro tests viz. amylase, hydrogen cyanide, lipase, catalase, protease and chitinase production confirmed the antagonistic efficacy of Variovorax soli. Nutrient acquisition enhancing efficiency of V. soli was established as positive results were recorded for PGP tests viz. siderophore production, nitrogen fixation, phosphate solubilization and exopolysaccharide production. Plants inoculated with V. soli induced phytohormonal modulation (viz. increase in IAA level and reduction in stress ethylene level) for enhanced shoot, root length, increased plant fresh and dry weight, chlorophyll and proline content for extensive root system, plant growth and alleviation of salt, drought, arsenic stress impact as compared to uninoculated plants, thus confirming its ability as biological fertilizers. Along with (IAA) indole 3-acetic acid and (ACC deaminase) 1-aminocyclopropane 1-carboxylic acid deaminase, certain other extracellular exudates of PGPR serve as signaling molecules to trigger abiotic stress responsive pathway. V. soli is therefore a promising green alternative to chemical fertilizers to induce abiotic stress tolerance and reclaim soil fertility to elevate agricultural productivity.
Variovorax soli 介导的辣椒生长改善和非生物胁迫缓解作用
本研究从土壤样本中分离出根瘤菌菌株,并在体外条件下对其促进植物生长和拮抗植物生长的潜力进行筛选。对表现出最大植物生长促进和拮抗潜力的分离株进行了生化鉴定和 16S rDNA 基因测序。根据分子特征鉴定,该分离物被确定为 Variovorax soli,NCBI登录号为 LC372534。Variovorax soli 的 PGP 潜力已得到证实,因为它能促进植物在对照和盐、干旱、重金属胁迫条件下的生长。体外测试(即淀粉酶、氰化氢、脂肪酶、过氧化氢酶、蛋白酶和几丁质酶的生产)的阳性结果证实了 Variovorax soli 的拮抗功效。在 PGP 试验(即苷元生产、固氮、磷酸盐溶解和外多糖生产)中,V. soli 的养分获取效率得到了证实。与未接种 V. soli 的植物相比,接种了 V. soli 的植物诱导了植物激素调节(即 IAA 水平的增加和胁迫乙烯水平的降低),从而增加了芽、根的长度,提高了植物的鲜重和干重,增加了叶绿素和脯氨酸含量,促进了广泛的根系、植物生长,并减轻了盐、干旱和砷胁迫的影响,从而证实了其作为生物肥料的能力。除了(IAA)吲哚-3-乙酸和(ACC 脱氨酶)1-氨基环丙烷-1-羧酸脱氨酶之外,PGPR 的某些其他胞外渗出物也是触发非生物胁迫响应途径的信号分子。因此,V. soli 是一种很有前途的绿色化肥替代品,可诱导非生物胁迫耐受性,恢复土壤肥力,提高农业生产力。
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来源期刊
Research Journal of Biotechnology
Research Journal of Biotechnology 工程技术-生物工程与应用微生物
CiteScore
0.60
自引率
0.00%
发文量
192
审稿时长
1.5 months
期刊介绍: We invite you to contribute Research Papers / Short Communications / Review Papers: -In any field of Biotechnology, Biochemistry, Microbiology and Industrial Microbiology, Soil Technology, Agriculture Biotechnology. -in any field related to Food Biotechnology, Nutrition Biotechnology, Genetic Engineering and Commercial Biotechnology. -in any field of Biotechnology related to Drugs and Pharmaceutical products for human beings, animals and plants. -in any field related to Environmental Biotechnolgy, Waste Treatment of Liquids, Soilds and Gases; Sustainability. -in inter-realted field of Chemical Sciences, Biological Sciences, Environmental Sciences and Life Sciences. -in any field related to Biotechnological Engineering, Industrial Biotechnology and Instrumentation. -in any field related to Nano-technology. -in any field related to Plant Biotechnology.
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