Assessment of the reliability of serological monitoring results based on biobank materials

Q3 Social Sciences

Cardiovascular Therapy and Prevention Pub Date : 2023-12-10 DOI:10.15829/1728-8800-2023-3709

A. Nozdracheva, T. A. Semenenko

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引用次数: 0

Abstract

Aim. To evaluate the safety of immunoglobulins (Ig) of different classes (IgA, IgM, IgG, IgE) under conditions of long-term low-temperature storage of blood serum samples.Material and methods. The work used samples of blood serum from the collection of the Department of Epidemiology, examined by enzyme immunoassay for antibodies of classes IgA, IgM, IgG, IgE twice as follows: immediately upon receipt in the laboratory and after storage at a temperature of -70о C for 8 years.Results. After the storage of seropositive sera, the level of IgA antibodies did not change significantly (p=0,7). For the remaining classes of immunoglobulins (IgM, IgG, IgE), a small (not >15%) but significant decrease (p<0,05), most pronounced for IgE, was observed. It was shown that the higher the antibody level in the samples during the first study, the more it decreased after long-term storage (correlation at p<0,05). This did not lead to false negative results, which is due to the greater accuracy in measuring small values.Conclusion. Long-term low-temperature storage of serum samples in biobank conditions ensures the safety of antibodies of different classes and is the basis for the reliability of future studies.

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根据生物库材料评估血清学监测结果的可靠性

目的评估血清样本长期低温储存条件下不同类别（IgA、IgM、IgG、IgE）免疫球蛋白（Ig）的安全性。这项工作使用了流行病学部收集的血清样本，用酶免疫测定法检测了 IgA、IgM、IgG、IgE 类抗体，检测过程分为两次：实验室收到样本后立即检测和在-70о C 温度下储存 8 年后检测。血清阳性血清储存后，IgA 抗体水平无明显变化（p=0.7）。其余类别的免疫球蛋白（IgM、IgG、IgE）的下降幅度很小（不大于 15%），但很明显（p<0,05），其中以 IgE 的下降最为明显。结果表明，第一次研究时样本中的抗体水平越高，长期储存后抗体水平下降越多（相关性为 p<0,05）。这并没有导致假阴性结果，这是因为测量小数值的准确性更高。在生物库条件下长期低温储存血清样本可确保不同类别抗体的安全性，是未来研究可靠性的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cardiovascular Therapy and Prevention Medicine-Cardiology and Cardiovascular Medicine

CiteScore

1.70

自引率

0.00%

发文量

155

审稿时长

6-12 weeks

期刊介绍： The most important objectives of the journal are: the generalization of scientific and practical achievements in the field of cardiology, increasing scientific and practical skills of cardiologists. The scientific concept of publication does the publication of modern achievements in the field of epidemiology, prevention and treatment of cardiovascular diseases, the results of research, national and international clinical trials. For publication in the journal are invited both domestic and foreign scientists and clinicians working in the field of cardiology, as well as doctors of other specialties. The magazine covers various issues in cardiology and related specialties. Each issue is prepared by Executive editor of the issue, a respected specialist in the field of epidemiology, prevention and treatment of cardiovascular diseases. The main focus of the publication — scientific articles on original research, the pharmacotherapy of cardiovascular disease, new diagnostic methods. All members of the group of authors should meet all four criteria of authorship set forth in the ICMJE recommendations: 1) concept and design development or data analysis and interpretation, and 2) manuscript justification or verification of critical intellectual content, and 3) final approval for publication of the manuscript, and 4) consent to be responsible for all aspects of the work, and assume that issues relating to the thoroughness and diligent execution of any part of the study submitted are duly investigated and resolved. Great importance the editors attached to the preparation of scientific papers by groups of authors at a high level, literacy, authors, and their ownership information, availability of research results not only to colleagues in Russia, but also abroad.