Phenolics-Enhancing Piper sarmentosum (Roxburgh) Extracts Pre-Treated with Supercritical Carbon Dioxide and its Correlation with Cytotoxicity and α-Glucosidase Inhibitory Activities

IF 0.6 Q3 MULTIDISCIPLINARY SCIENCES

Pertanika Journal of Science and Technology Pub Date : 2023-12-21 DOI:10.47836/pjst.32.1.18

M. S. R. Hamil, Fauziahanim Zakaria, Lee Chong Yew, Norzilawati Pauzi, K. S. Mohd, N. H. Saidan

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引用次数: 0

Abstract

Piper sarmentosum was found to have various phytochemicals such as polyphenols and flavonoids but also contains asarone isomers, which were reported as carcinogenic in several preclinical studies. Therefore, reducing asarone isomers from this functional food is important while retaining its functional properties. This study compares the total phenolics, total flavonoids, vitexin and naringenin contents, cytotoxicity, and anti-hyperglycaemic activity between various solvent extracts using a two-step extraction with SC-CO2 pre-treatment and a single-step conventional solvent extraction without SC-CO2 pre-treatment. The findings showed that phenolic content was significantly enhanced in ethanol, 50% ethanol, and water extracts pre-treated with SC-CO2, significantly correlated with α-glucosidase inhibitory activity. SC-CO2 pre-treated extracts enhanced the viability of two normal fibroblasts NIH/3T3 and CCD-18Co cell lines. It is concluded that SC-CO2 extraction offers a rapid pre-treatment step to produce safer extracts with better quality and efficacy.

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用超临界二氧化碳预处理的酚类增强型胡椒（罗克斯堡）提取物及其与细胞毒性和α-葡萄糖苷酶抑制活性的相关性

研究发现，瓜蒌中含有多种植物化学物质，如多酚和类黄酮，但也含有asarone 异构体，据几项临床前研究报告，asarone 异构体具有致癌性。因此，在保留其功能特性的同时，减少这种功能性食品中的asarone 异构体含量非常重要。本研究比较了经 SC-CO2 预处理的两步提取法和未经 SC-CO2 预处理的单步传统溶剂提取法提取的各种溶剂提取物的总酚类、总黄酮类、牡荆素和柚皮苷含量、细胞毒性和抗高血糖活性。研究结果表明，经 SC-CO2 预处理的乙醇提取物、50% 乙醇提取物和水提取物中的酚含量明显提高，这与α-葡萄糖苷酶的抑制活性显著相关。经 SC-CO2 预处理的提取物可增强 NIH/3T3 和 CCD-18Co 两种正常成纤维细胞的活力。结论是，SC-CO2 萃取提供了一个快速预处理步骤，可生产出更安全、质量更好、功效更高的提取物。

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来源期刊

Pertanika Journal of Science and Technology MULTIDISCIPLINARY SCIENCES-

CiteScore

1.50

自引率

16.70%

发文量

178

期刊介绍： Pertanika Journal of Science and Technology aims to provide a forum for high quality research related to science and engineering research. Areas relevant to the scope of the journal include: bioinformatics, bioscience, biotechnology and bio-molecular sciences, chemistry, computer science, ecology, engineering, engineering design, environmental control and management, mathematics and statistics, medicine and health sciences, nanotechnology, physics, safety and emergency management, and related fields of study.